Results 21 to 30 of about 370,485 (298)

Enzymatic activity of bromelain isolated pineapple (Ananas comosus) hump and its antibacterial effect on Enterococcus faecalis

Scientific Dental Journal, 2018
Background: Enterococcus faecalis is the frequent cause of oral infections, such as periodontitis, infected root canals, and peri-radicular abscesses. Pineapple (Ananas comosus) fruit contains bromelain, one of proteolytic enzymes associated with several
Dewi Liliany, Armelia Sari Widyarman, Erni Erfan, Janti Sudiono, Melanie S Djamil   +4 more
doaj   +1 more source

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Journal of Visualized Experiments, 2017
Biological small angle X-ray scattering (BioSAXS) is a powerful technique in molecular and structural biology used to determine solution structure, particle size and shape, and surface-to-volume ratio of macromolecules.
M. Brennich, A. Round, Stephanie Hutin
semanticscholar   +1 more source

Online ion-exchange chromatography for small-angle X-ray scattering

Acta Crystallographica Section D: Structural Biology, 2016
SAXS coupled with online ion-exchange chromatography allows the collection of high-quality BioSAXS data.
Stephanie Hutin, M. Brennich, Benoît Maillot, A. Round   +3 more
semanticscholar   +1 more source

A competitive binding-mass spectrometry strategy for high-throughput evaluation of potential critical quality attributes of therapeutic monoclonal antibodies

mAbs, 2022
Therapeutic monoclonal antibodies (mAbs) have a propensity to host a large number of chemical and enzymatical modifications that need to be properly assessed for their potential impact on target binding.
Zhengqi Zhang, Yuetian Yan, Shunhai Wang, Ning Li   +3 more
doaj   +1 more source

Three dimensional liquid chromatography coupling ion exchange chromatography/hydrophobic interaction chromatography/reverse phase chromatography for effective protein separation in top-down proteomics.

Analytical Chemistry, 2015
To address the complexity of the proteome in mass spectrometry (MS)-based top-down proteomics, multidimensional liquid chromatography (MDLC) strategies that can effectively separate proteins with high resolution and automation are highly desirable ...
Santosh G. Valeja, Lichen Xiu, Zachery R. Gregorich, Huseyin Guner, Song Jin, Ying Ge   +5 more
semanticscholar   +1 more source

Hydrophobicity of polymer based anion-exchange columns for ion chromatography

Heliyon, 2021
The regularities of the retention of alkanoic and alkanesulfonic acids homologues were investigated for the set of 36 anion-exchange columns produced by various manufacturers.
Pavel N. Nesterenko, Ekaterina P. Nesterenko   +1 more
doaj  

Isotope effects of neodymium in different ligands exchange systems studied by ion exchange displacement chromatography

Journal of Advanced Research, 2013
The isotope effects of neodymium in Nd-glycolate ligand exchange system were studied by using ion exchange chromatography. The separation coefficients of neodymium isotopes, ε’s, were calculated from the observed isotopic ratios at the front and rear ...
Ibrahim Ismail, Ahmed S. Fawzy, Mohammad I. Ahmad, Hisham F. Aly, Masao Nomura, Yasuhiko Fujii   +5 more
doaj   +1 more source

Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]

Advanced Pharmaceutical Bulletin, 2015
Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi, Jafar Majidi, Leili Aghebati Maleki, Jalal Abdolalizadeh, Mehdi Yousefi, Majid Ahmadi, Somayeh Dadashi, Zahra Moradi, Elmira Zolali   +8 more
doaj   +1 more source

Nanoscale Ion-Exchange Materials: From Analytical Chemistry to Industrial and Biomedical Applications

Molecules, 2023
Nano-sized ion exchangers (NIEs) combine the properties of common bulk ion-exchange polymers with the unique advantages of downsizing into nanoparticulate matter.
Magdalena Matczuk, Lena Ruzik, Bernhard K. Keppler, Andrei R. Timerbaev   +3 more
doaj   +1 more source

Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae

Glycobiology, 2020
Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs. Standardized work with fucoidans is required for structure–function studies, but remains
Andreas Sichert, S. Le Gall, L. Klau, B. Laillet, H. Rogniaux, F. Aachmann, J. Hehemann   +6 more
semanticscholar   +1 more source