Results 21 to 30 of about 105,791 (260)
Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence
Molecular Oncology, EarlyView.The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova +18 more
wiley +1 more source
Hydrophobicity of polymer based anion-exchange columns for ion chromatography
Heliyon, 2021 The regularities of the retention of alkanoic and alkanesulfonic acids homologues were investigated for the set of 36 anion-exchange columns produced by various manufacturers.
Pavel N. Nesterenko +1 more
doaj
Formation of dimers in refolding of recombinant human interferon-γ by ion-exchange chromatography
浙江大学学报. 农业与生命科学版, 2006 The refolding process was performed by gradually decreasing the concentration of urea in the buffer after the denatured rhIFN-γ proteins had been bound onto the ion-exchange gel SP Sepharose Fast Flow.
JIN Ting, GUAN Yi-xin, YAO Shan-jing
doaj +1 more source
mAbs, 2022 Therapeutic monoclonal antibodies (mAbs) have a propensity to host a large number of chemical and enzymatical modifications that need to be properly assessed for their potential impact on target binding.
Zhengqi Zhang +3 more
doaj +1 more source
A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner
Molecular Oncology, EarlyView.We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp +13 more
wiley +1 more source
Journal of Advanced Research, 2013 The isotope effects of neodymium in Nd-glycolate ligand exchange system were studied by using ion exchange chromatography. The separation coefficients of neodymium isotopes, ε’s, were calculated from the observed isotopic ratios at the front and rear ...
Ibrahim Ismail +5 more
doaj +1 more source
Molecules, 2023 Nano-sized ion exchangers (NIEs) combine the properties of common bulk ion-exchange polymers with the unique advantages of downsizing into nanoparticulate matter.
Magdalena Matczuk +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo +4 more
wiley +1 more source
Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]
Advanced Pharmaceutical Bulletin, 2015 Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi +8 more
doaj +1 more source
Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli
FEBS Open Bio, EarlyView.BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa +2 more
wiley +1 more source