Results 41 to 50 of about 120,195 (208)
Journal of Lipid Research, 1977 A method is described for quantitative analysis of bile acids in urine. Urine is acidified and bile acids are extracted on an Amberlite XAD-2 column.
B Almé +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
FEBS Open Bio, EarlyView.Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Jurnal Teknologi dan Industri Pangan, 2015 Phosphorus is a critical dietary mineral that is involved as phosphate in bone, tooth, nucleic acids or phospholipids and is mainly supplied through consumption of dairy products.
Tia Amina Setiawati +3 more
doaj +1 more source
Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]
Advanced Pharmaceutical Bulletin, 2015 Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi +8 more
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FEBS Open Bio, EarlyView.Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada +7 more
wiley +1 more source
CompoundsCyclic trimetaphosphate (cTmp) has been used as a prebiotically plausible compound to generate chemically activated nucleoside 5′-phosphates. While cTmp is mainly available as a trisodium salt, some experiments benefit from different cations.
Ulrich F. Müller
doaj +1 more source
Morphology, Transport, and Dynamics of Protein Adsorption in Open‐Cell Metal Foam
Advanced Engineering Materials, EarlyView.Stainless steel (SS) open‐cell foams are shown to adsorb more protein per unit area than previously reported 316L SS and chromium oxide surfaces under static and flow conditions. An integrated approach combining 3D pore imaging, flow simulation, and protein adsorption experiments characterizes the foam’s performance.
Chinmaya Prerana Inguva +2 more
wiley +1 more source
Isomorphous Substitution of P(V) in Natural Clinoptilolite: Evalution of the Product for Removal of NO3-, NO2- and F - from Aqueous Solutions [PDF]
Iranian Journal of Chemistry & Chemical Engineering, 2008 Nitrate is an anion of major importance particularly in biological areas. Excessive amounts in water supplies indicate pollution from sewage or agricultural effluents. The effects of excessive quantities of nitrate in water are well known.
Hossein Faghihian +1 more
doaj
Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption
Advanced Functional Materials, EarlyView.Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy +7 more
wiley +1 more source