Results 21 to 30 of about 125,999 (289)

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source

Antimicrobial activity of endophytic fungi Kabatiella caulivora Var. A from Alyxia reinwardtii Bl.

Journal of Biological Researches, 2012
Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7.
Y. Sri Wulan Manuhara
doaj   +1 more source

Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography

Molecular Therapy: Methods & Clinical Development, 2019
Lentiviral vectors (LVs) are used in cell and gene therapies due to their ability to transduce both dividing and non-dividing cells while carrying a relatively large genetic payload and providing long-term gene expression via gene integration.
Jelena Ruscic, Christopher Perry, Tarit Mukhopadhyay, Yasu Takeuchi, Daniel G. Bracewell   +4 more
doaj   +1 more source

Study on separation and purification process of C1 esterase inhibitor

Zhongguo shuxue zazhi, 2022
Objective To study the technology of separating and purifying C1 esterase inhibitor (C1-INH) by using the waste washing liquid as raw materia during the preparation of human prothrombin complex (PCC) l.
Jiabin XU, Yawen LI, Jiali GONG, Erhua LUO, Meng ZHANG   +4 more
doaj   +1 more source

Testing of Ion Exchange Chromatography Media for Extraterrestrial in situ Sample Preparation on Liquid Samples

, 2022
Analysis of liquid samples often includes an initial sample preparation step in which components that are not of interest, or other interfering substances can be removed. Concentration of analytes in a smaller volume is also often important.
Jerome, Ferrance
core   +1 more source

Silent hemoglobin variant during capillary electrophoresis: A case report

Journal of Diabetes Investigation, 2020
Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes.
Yanping Yuan, Xianghai Zhou, Leili Gao, Qian Ren, Linong Ji   +4 more
doaj   +1 more source

Ion exchange chromatography protocol.

, 2023
Schematic and summarized protocol for the isolation of sEVs from conditioned media by ion exchange chromatography.
Natalia Pacienza (4669699), Gustavo Yannarelli (4669696), Anna Maria Tolomeo (10134020), Ricardo Malvicini (10588781), Diego Santa-Cruz (4669702), Maurizio Muraca (467702)   +5 more
core   +1 more source

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Extraction and Purification of albumin from Hunan plasma

مجلة بغداد للعلوم, 2011
This study includes adescription of Human serum Albumin by amodified using ion- exchange chromatography with manipulated comparison with cold ethanol precipitation method , It has been nticed that this procedure is superior orer the classical method ...
Baghdad Science Journal
doaj   +1 more source

Separation of Natural Collagen Crosslinks Using Buffer and Ion-pairing Agent Free Solvents on Silica Hydride Column for Mass Spectrometry Detection

Bio-Protocol, 2019
In this protocol we describe the separation of collagen crosslinks in biological tissues and samples including skin, tendon, cartilage, bone and urine. The existing methods use either cation exchange chromatography followed by post-column derivatization ...
Rafea Naffa, Joseph Pesek
doaj   +1 more source