Results 91 to 100 of about 959,191 (335)

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

An R package to analyse LC/MS metabolomic data: MAIT (Metabolite Automatic Identification Toolkit) [PDF]

, 2017
Current tools for liquid chromatography and mass spectrometry for metabolomic data cover a limited number of processing steps, whereas online tools are hard to use in a programmable fashion. This article introduces the Metabolite Automatic Identification
Andrés Lacueva, Ma. Cristina, Fernández Albert, Francesc, Llorach, Rafael, Perera Lluna, Alexandre   +3 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Recent developments in the rapid analysis of plants and tracking their bioactive constituents [PDF]

, 2009
Natural products chemistry has witnessed many new developments in the last 5 years like extractions with subcritical water and ionic liquids, LC/HRMS and LC/SPE/cryo-NMR, UHPLC, TLC/MS, MS-based preparative HPLC, comprehensive chromatography (GC × GC, LC
Beek, T.A., van, Claassen, F.W., Dapkevicius, A., Exarchou, V., Jeurissen, S.M.F., Klift, E.J.C., van der, Koleva, I., Tetala, K.K.R.   +7 more
core   +2 more sources

Headspace Solid-Phase Microextraction/Gas Chromatography–Mass Spectrometry for the Determination of 2-Nonenal and Its Application to Body Odor Analysis [PDF]

, 2021
Keita Saito, Yoshiyuki Tokorodani, Chihiro Sakamoto, Hiroyuki Kataoka   +3 more
openalex   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Gas Chromatography-Mass Spectrometry [PDF]

, 1971
In choosing a method by which to identify drug metabolites, the quantity of material which is available is a prime consideration. In this regard, pharmacologists find themselves midway between natural products chemists and biochemists. Usually the former has reasonably large quantities of material available in a relatively high state of purity.
A. M. Guarino, H. M. Fales
openaire   +1 more source

MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis. [PDF]

, 2015
Data-independent acquisition (DIA) in liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) provides comprehensive untargeted acquisition of molecular data.
Arita, Masanori, Cajka, Tomas, Fiehn, Oliver, Higgins, Brendan, Ikeda, Kazutaka, Kanazawa, Mitsuhiro, Kind, Tobias, Ma, Yan, Tsugawa, Hiroshi, VanderGheynst, Jean   +9 more
core  

Diamondoid diacids ('O4' species) in oil sands process-affected water. [PDF]

, 2013
RATIONALE: As a by-product of oil sands extraction, large volumes of oil sands process water (OSPW) are generated, which are contaminated with a large range of water-soluble organic compounds.
Barrow, Beam, Clemente, Frank, Frank, Grewer, Han, Han, Headley, Headley, Jones, Leishman, Nemba, Rontani, Rowland, Rowland, Rowland, Rowland, Rowland, Scarlett, Waltman, Wang   +21 more
core   +2 more sources

Discovery and Targeted Proteomic Studies Reveal Striatal Markers Validated for Huntington's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Clinical trials for Huntington's disease (HD) enrolling persons before clinical motor diagnosis (CMD) lack validated biomarkers. This study aimed to conduct an unbiased discovery analysis and a targeted examination of proteomic biomarkers scrutinized by clinical validation. Methods Cerebrospinal fluid was obtained from PREDICT‐HD and
Daniel Chelsky, Cara Joyce, H. Jeremy Bockholt, Paul A. Rudnick, William H. Adams, Fiona McAllister, Justin W. Smock, Michael A. Newton, Jane S. Paulsen   +8 more
wiley   +1 more source