Results 41 to 50 of about 3,141,961 (402)

Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. [PDF]

, 2018
Correct folding and post-translational modifications are vital for therapeutic proteins to elicit their biological functions. Osteopontin (OPN), a bone regenerative protein present in a range of mammalian cells, is an acidic phosphoprotein with multiple ...
Guo, Shengchun, Mayfield, Stephen, Nikolov, Zivko L, Rasala, Beth, Ravi, Ayswarya, Tran, Miller   +5 more
core   +2 more sources

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Applicability and Limitations of a Capillary-LC Column-Switching System Using Hybrid Graphene-Based Stationary Phases

Molecules, 2023
Graphene oxide sheets fixed over silica particles (SiGO) and their modification functionalized with C18 and endcapped (SiGO-C18ec) have been reported as sorbents for extraction and analytical columns in LC.
João Victor Basolli Borsatto, Edvaldo Vasconcelos Soares Maciel, Alejandro Cifuentes, Fernando Mauro Lanças   +3 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Development and Validation of a Novel LC-MS/MS Method for a TDM-Guided Personalization of HSCT Conditioning with High-Dose Busulfan in Children

Biomedicines, 2023
Personalization of busulfan (Bu) exposure via therapeutic drug monitoring (TDM) is recommended for patients treated with high-dose conditioning regimens.
Alessia Cafaro, Federica Pigliasco, Giammarco Baiardi, Sebastiano Barco, Manuela Stella, Roberto Bandettini, Francesca Mattioli, Maura Faraci, Giuliana Cangemi   +8 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Combining ion chromatography with mass spectrometry and inductively coupled plasma‐mass spectrometry: Annual review 2020

Analytical Science Advances, 2021
The demand for analyzing low molecular weight polar and ionic components in body fluids, pharmaceutical formulations, food, environmental samples, and drinking water is increasing. Ion chromatography (IC) offers significant advantages over RPLC and HILIC
Cees Bruggink, Detlef Jensen
doaj   +1 more source

Preliminary qualitative screening for cancer chemopreventive agents in Telfairia occidentalis Hook.f., Gnetum africanum Welw., Gongronema latifolium Benth. and Ocimum gratissimum L. from Nigeria [PDF]

, 2009
The leaves of four plant foods commonly consumed in Nigeria namely Gnetum africanum (Igbo name: Ukazi), Gongronema latifolium (Igbo name: Utazi), Telfairia occidentalis (Igbo name: Ugu), Ocimum gratissimum (Igbo name: Nchoanwu), were each screened for ...
Iweala, E. E. J.
core  

Identifying metabolites by integrating metabolome databases with mass spectrometry cheminformatics. [PDF]

, 2018
Novel metabolites distinct from canonical pathways can be identified through the integration of three cheminformatics tools: BinVestigate, which queries the BinBase gas chromatography-mass spectrometry (GC-MS) metabolome database to match unknowns with ...
A El-Tayeb, AD Hanson, AL Hartman, Atsushi Ogiwara, C Fattuoni, C Ruttkies, CL Linster, DY Lee, F Allen, Gert Wohlgemuth, GJ Patti, H Sperber, H Tsugawa, H Tsugawa, H Tsugawa, HD Flosadóttir, Hiroshi Tsugawa, I Yamamoto, J Budczies, JG Jeffryes, John Meissen, K Haug, Kohei Takeuchi, M Sud, Masanori Arita, Matthew Mueller, Megan Showalter, MP Styczynski, MR Showalter, O Fiehn, O Fiehn, O Fiehn, O Khersonsky, Oliver Fiehn, Peter Beal, RR da Silva, S Kim, S Kumari, Sajjan Mehta, SE Stein, SM Rappaport, T Kind, Tobias Kind, WR Wikoff, Yuxuan Zheng, Zijuan Lai   +45 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source