Results 91 to 100 of about 1,560,009 (266)
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Background The clinical relevance of MGMT promoter methylation in IDH‐mutant gliomas remains controversial in the era of molecular classification. We aimed to systematically evaluate its clinical relevance by integrating quantitative assessment, cutoff exploration, and adjustment for clinical confounding.
Haihui Jiang +7 more
wiley +1 more source
Chromosome Conformation Capture
, 2014 Author: Marcus Marvin ### Nuclei preparation **Preparing the cells** 1. 300–500 ml cultures of density 1.5–4.0 x 10e7 cells/ml - Fix cells with freshly prepared formaldehyde in Buffer A for 2 minutes, stirring, to a final concentration of 1% - Quench fixation by adding glycine to final concentration of 0.125 M - Wash cells twice in Buffer A ...
openaire +2 more sources
Mating type specific chromosome conformation in [PDF]
, 2013 Budding yeast switch their mating type by a gene conversion event at the MAT locus which uses either of two silent loci (HML or HMR) on opposite ends of chromosome three as a template. InMATa cells the left arm of Chr. Ill is “activated” which allows for
Dekker, Job +5 more
core +1 more source
Disentangling the Genetic Relationship Between Sleep and Epilepsy
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective A bidirectional relationship between sleep and epilepsy is well recognised, yet the biological mechanisms linking them remain poorly understood. Both have a genetic basis, with genome‐wide association studies (GWAS) capturing polygenic contributions.
Khang N. Le +9 more
wiley +1 more source
HiCapTools: a software suite for probe design and proximity detection for targeted chromosome conformation capture applications. [PDF]
Bioinformatics, 2018 Summary Folding of eukaryotic genomes within nuclear space enables physical and functional contacts between regions that are otherwise kilobases away in sequence space.
Anil A +3 more
europepmc +2 more sources
Advanced Healthcare Materials, EarlyView.Recent advances in microfluidic technologies for microgel fabrication are critically reviewed, highlighting the capabilities and limitations of major microfluidic platforms and discussing how platform design, material selection, and crosslinking strategies influence microgel architecture and functionality. Current challenges and future opportunities in
Afshin Abrishamkar +6 more
wiley +1 more source
An integrated model for detecting significant chromatin interactions from high-resolution Hi-C data
Nature Communications, 2017 Genome-wide chromosome conformation capture has helped us identify features of genome topology influencing biology but requires careful statistical analysis.
Mark Carty +6 more
doaj +1 more source
Measuring Blood Plasma Glutamine Levels Using a Nanopore‐Based Sensor
Advanced Healthcare Materials, EarlyView.Glutamine (GLN) is a biomarker useful for predicting toxic ammonia accumulation. Here, we used a biosensor based on the Cytolysin A (ClyA) nanopore coupled with the GLN‐specific protein substrate‐binding domain 2 (SBD2) to demonstrate real‐time measurement of GLN in blood plasma, highlighting the potential for future point‐of‐care applications ...
Sofia B. Relvas +9 more
wiley +1 more source
Advanced Science, EarlyView.This study identified Danofloxacin as a novel supporter of the maintenance of mouse embryonic stem cell (ESC) pluripotency. It functions partially by inhibiting HDAC1, which increases specific histone acetylation marks and activates key genes like Tert and Prdm10.
Yan Zhang +8 more
wiley +1 more source
, 2018 Mammalian genomes are folded in a hierarchy of compartments, topologically associating domains (TADs), subTADs and looping interactions. Currently, there is a great need to evaluate the link between chromatin topology and genome function across many ...
Wanfeng Gong +5 more
core +1 more source