Microfluidics-Based Chromosome Conformation Capture (3C) Technology for Examining Chromatin Organization with a Low Quantity of Cells. [PDF]
Anal Chem, 2018 Detecting three-dimensional (3D) genome organization in the form of physical interactions between various genomic loci is of great importance for understanding transcriptional regulations and cellular fate.
Sun C, Lu C.
europepmc +7 more sources
Metagenomic Chromosome Conformation Capture (3C): techniques, applications, and challenges. [PDF]
F1000Res, 2015 We review currently available technologies for deconvoluting metagenomic data into individual genomes that represent populations, strains, or genotypes present in the community. An evaluation of chromosome conformation capture (3C) and related techniques
Liu M, Darling A.
europepmc +6 more sources
Deconvoluting simulated metagenomes: the performance of hard- and soft- clustering algorithms applied to metagenomic chromosome conformation capture (3C). [PDF]
PeerJ, 2016 © 2016 DeMaere and Darling. Background. Chromosome conformation capture, coupled with high throughputDNA sequencing in protocols like Hi-C and 3C-seq, has been proposed as a viable means of generating data to resolve the genomes of microorganisms living ...
DeMaere MZ, Darling AE.
europepmc +12 more sources
Quantitative Chromosome Conformation Capture (3C-qPCR)
Methods in Molecular Biology, 2022 International audienceMany population-based methods investigating chromatin dynamics and organization in eukaryotes are based on the Chromosome Conformation Capture (3C) method.
Thierry Forné
exaly +6 more sources
Metagenome Analysis Exploiting High-Throughput Chromosome Conformation Capture (3C) Data.
Trends Genet, 2015 International audienceMicrobial communities are complex and constitute important parts of our environment. Genomic analysis of these populations is a dynamic research area but remains limited by the difficulty in assembling full genomes of individual ...
Marbouty M, Koszul R.
europepmc +7 more sources
Studying physical chromatin interactions in plants using Chromosome Conformation Capture (3C). [PDF]
Nature Protocols, 2009 Gene regulation in higher eukaryotes frequently involves physical interactions between genomic sequence elements tens of kilobases apart on the same chromosome but can also entail interactions between different chromosomes.
Louwers, M. +9 more
core +6 more sources
Quantitative analysis of chromosome conformation capture assays (3C-qPCR) [PDF]
Nature Protocols, 2011 Chromosome conformation capture (3C) technology is a pioneering methodology that allows in vivo genomic organization to be explored at a scale encompassing a few tens to a few hundred kilobase-pairs.
Splinter, Erik +7 more
core +6 more sources
Mapping cis- and trans- chromatin interaction networks using chromosome conformation capture (3C). [PDF]
Methods Mol Biol, 2009 Expression of genes can be controlled by regulatory elements that are located at large genomic distances from their target genes (in cis), or even on different chromosomes (in trans). Regulatory elements can act at large genomic distances by engaging in direct physical interactions with their target genes resulting in the formation of chromatin loops ...
Miele A, Dekker J.
europepmc +7 more sources
5C-ID: Increased resolution Chromosome-Conformation-Capture-Carbon-Copy with in situ 3C and double alternating primer design [PDF]
Methods, 2018 Mammalian genomes are folded in a hierarchy of compartments, topologically associating domains (TADs), subTADs and looping interactions. Currently, there is a great need to evaluate the link between chromatin topology and genome function across many ...
Wanfeng Gong +5 more
core +7 more sources
Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification
, 2009 International audienceChromosome conformation capture (3C) methodology was developed to study spatial organization of long genomic regions in living cells.
Eivazova, Elvira +11 more
core +5 more sources