The macro and micro of chromosome conformation capture [PDF]
Wiley Interdisciplinary Reviews: Developmental Biology, 2021 AbstractThe 3D organization of the genome facilitates gene regulation, replication, and repair, making it a key feature of genomic function and one that remains to be properly understood. Over the past two decades, a variety of chromosome conformation capture (3C) methods have delineated genome folding from megabase‐scale compartments and topologically
Anders S Hansen, Viraat Goel
exaly +6 more sources
Orchestrating chromosome conformation capture analysis with Bioconductor [PDF]
Nature CommunicationsGenome-wide chromatin conformation capture assays provide formidable insights into the spatial organization of genomes. However, due to the complexity of the data structure, their integration in multi-omics workflows remains challenging.
Jacques Serizay +4 more
doaj +5 more sources
A cautionary note on the use of chromosome conformation capture in plants [PDF]
Plant Methods, 2017 Background The chromosome conformation capture (3C) technique is a method to study chromatin interactions at specific genomic loci. Initially established for yeast the 3C technique has been adapted to plants in recent years in order to study chromatin ...
Suraj Jamge +3 more
doaj +6 more sources
Investigation of the Basic Steps in the Chromosome Conformation Capture Procedure [PDF]
Frontiers in Genetics, 2021 A constellation of chromosome conformation capture methods (С-methods) are an important tool for biochemical analysis of the spatial interactions between DNA regions that are separated in the primary sequence.
Oleg V. Bylino +5 more
doaj +2 more sources
Chromosome conformation capture technologies as tools to detect structural variations and their repercussion in chromatin 3D configuration [PDF]
Frontiers in Cell and Developmental Biology, 2023 3D genome organization regulates gene expression in different physiological and pathological contexts. Characterization of chromatin structure at different scales has provided information about how the genome organizes in the nuclear space, from ...
Aura Stephenson-Gussinye +1 more
doaj +2 more sources
CiFi: accurate long-read chromosome conformation capture with low-input requirements [PDF]
Nature CommunicationsHi-C characterizes three-dimensional chromatin organization, facilitates haplotype phasing, and enables genome-assembly scaffolding, but encounters difficulties across complex regions.
Sean P. McGinty +10 more
doaj +3 more sources
Chromosome conformation capture assay combined with biotin enrichment for hyperthermophilic archaea [PDF]
STAR Protocols, 2021 Summary: Chromosome organization in archaea has long been enigmatic due, in part, to the typically small cell size of archaea and the extremophilic nature of many of the model archaeal species studies, rendering live-cell imaging technically challenging.
Naomichi Takemata, Stephen D. Bell
doaj +2 more sources
Chromosome conformation capture approaches to investigate 3D genome architecture in Ankylosing Spondylitis [PDF]
Frontiers in Genetics, 2023 Ankylosing Spondylitis (AS) is a chronic inflammatory arthritis of the spine exhibiting a strong genetic background. The mechanistic and functional understanding of the AS-associated genomic loci, identified with Genome Wide Association Studies (GWAS ...
Connor Davidson +7 more
doaj +2 more sources
Generating Chromosome Geometries in a Minimal Cell From Cryo-Electron Tomograms and Chromosome Conformation Capture Maps [PDF]
Frontiers in Molecular Biosciences, 2021 JCVI-syn3A is a genetically minimal bacterial cell, consisting of 493 genes and only a single 543 kbp circular chromosome. Syn3A’s genome and physical size are approximately one-tenth those of the model bacterial organism Escherichia coli’s, and the ...
Benjamin R. Gilbert +9 more
doaj +2 more sources
Systematic evaluation of chromosome conformation capture assays. [PDF]
Nat Methods, 2021 Abstract Chromosome conformation capture (3C)-based assays are used to map chromatin interactions genome-wide. Quantitative analyses of chromatin interaction maps can lead to insights into the spatial organization of chromosomes and the mechanisms by which they fold.
Akgol Oksuz B +15 more
europepmc +7 more sources