Results 41 to 50 of about 20,695 (239)
Application of Hi-C and other omics data analysis in human cancer and cell differentiation research
Computational and Structural Biotechnology Journal, 2021 With the development of 3C (chromosome conformation capture) and its derivative technology Hi-C (High-throughput chromosome conformation capture) research, the study of the spatial structure of the genomic sequence in the nucleus helps researchers ...
Haiyan Gong +4 more
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3PD: Rapid design of optimal primers for chromosome conformation capture assays
BMC Genomics, 2009 Background Higher eukaryotes control the expression of their genes by mechanisms that we are just beginning to understand. A complex layer of control is the dynamic spatial organization of the nucleus.
Dieterich Christoph, Fröhler Sebastian
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Capturing Chromosome Conformation Across Length Scales
Journal of Visualized Experiments, 2023 Chromosome conformation capture (3C) is used to detect three-dimensional chromatin interactions. Typically, chemical crosslinking with formaldehyde (FA) is used to fix chromatin interactions. Then, chromatin digestion with a restriction enzyme and subsequent religation of fragment ends converts three-dimensional (3D) proximity into unique ligation ...
Yang, Liyan +3 more
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Chromosome conformation capture uncovers potential genome-wide interactions between human conserved non-coding sequences. [PDF]
PLoS ONE, 2011 Comparative analyses of various mammalian genomes have identified numerous conserved non-coding (CNC) DNA elements that display striking conservation among species, suggesting that they have maintained specific functions throughout evolution.
Daniel Robyr +10 more
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Chromosome Conformation Capture (3C) in Budding Yeast [PDF]
Cold Spring Harbor Protocols, 2015 Chromosome conformation capture (3C) is a method for studying chromosomal organization that takes advantage of formaldehyde cross-linking to measure the spatial association of two pieces of chromatin. The 3C method begins with whole-cell formaldehyde fixation of chromatin.
Belton, Jon-Matthew, Dekker, Job
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BioTechniques, 2015 Three-dimensional (3-D) genome organization in the nuclear space affects various genomic functions. Circular chromosome conformation capture (4C-seq) is a powerful technique that allows researchers to measure long-range chromosomal interactions with a ...
Michal Schwartz +7 more
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BMC Research Notes, 2022 Objectives Transcriptional regulatory modules are usually modelled via a network, in which nodes correspond to genes and edges correspond to regulatory associations between them.
Thibault Poinsignon +4 more
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Simultaneous Profiling of Chromosome Conformation and Gene Expression in Single Cells
Bio-Protocol, 2023 Rapid development in single-cell chromosome conformation capture technologies has provided valuable insights into the importance of spatial genome architecture for gene regulation.
Yujie Chen +3 more
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Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
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Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source