Randomized ligation control for chromosome conformation capture
Cold Spring Harbor Protocols, 2015 In experiments using chromosome conformation capture followed by PCR (3C-PCR) or chromosome conformation capture carbon copy (5C), it is critical to control for intrinsic biases in the restriction fragments of interest and the probes or primers used for ...
Dekker, Job, Belton, Jon-Matthew
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Mapping chromatin interactions by chromosome conformation capture
Current Protocols in Molecular Biology, 2011 Chromosome conformation capture (3C) is one of the only techniques that allows for analysis of an intermediate level of chromosome structure ranging from a few to hundreds of kilobases, a level most relevant for gene regulation.
Dekker, Job +4 more
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A cautionary note on the use of chromosome conformation capture in plants [PDF]
Plant Methods, 2017 Background The chromosome conformation capture (3C) technique is a method to study chromatin interactions at specific genomic loci. Initially established for yeast the 3C technique has been adapted to plants in recent years in order to study chromatin ...
Suraj Jamge +3 more
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Application of Chromosome Conformation Capture Method for Detection MYC/TRD Chromosomal Translocation in Leukemia Cell Line [PDF]
International Journal of Hematology-Oncology and Stem Cell Research, 2020 Background: Chromosomal breakpoints are the most common cause of hereditary diseases and cancers. Today, many standard clinical methods such as cytogenetic and PCR based techniques are used which have limitation regarding detection resolution. Chromosome
Moloud Absalan +7 more
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Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture. [PDF]
Nat Protoc, 2022 Chromosome conformation capture (3C) methods measure the spatial proximity between DNA elements in the cell nucleus. Many methods have been developed to sample 3C material, including the Capture-C family of protocols.
Downes DJ +9 more
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Chromosome conformation capture in primary human cells
, 2016 3D organization of the genome, its structural and regulatory function of cell identity, is acquiring prominent features in epigenetics studies; more efforts have been done to develop techniques that allow studying nuclear structure.
Bodega B., Cortesi A.
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Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies [PDF]
Horticulture Research, 2018 Genomes: a new map of field mustard A detailed third-generation genome sequence has been published for Brassica rapa, which includes crops such as napa cabbage, turnips, and bok choy.
Lei Zhang +19 more
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Computational Methods for Predicting 3D Genomic Organization From High-Resolution Chromosome Conformation Capture Data [PDF]
Briefings in Functional Genomics, 2020 Kimberly MacKay, Anthony J. Kusalik
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High-resolution circular chromosome conformation capture assay
Nature Protocols, 2008 The pioneering chromosome conformation capture (3C) method provides the opportunity to study chromosomal folding in the nucleus. It is based on formaldehyde cross-linking of living cells followed by enzyme digestion, intramolecular ligation and ...
Gondor, A +8 more
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The DLO Hi-C Tool for Digestion-Ligation-Only Hi-C Chromosome Conformation Capture Data Analysis. [PDF]
Genes (Basel), 2020 It is becoming increasingly important to understand the mechanism of regulatory elements on target genes in long-range genomic distance. 3C (chromosome conformation capture) and its derived methods are now widely applied to investigate three-dimensional (
Hong P +6 more
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