Results 51 to 60 of about 1,497,962 (254)

Capturing Chromosome Conformation Across Length Scales

Journal of Visualized Experiments, 2023
Chromosome conformation capture (3C) is used to detect three-dimensional chromatin interactions. Typically, chemical crosslinking with formaldehyde (FA) is used to fix chromatin interactions. Then, chromatin digestion with a restriction enzyme and subsequent religation of fragment ends converts three-dimensional (3D) proximity into unique ligation ...
Yang, Liyan, Akgol Oksuz, Betul, Dekker, Job, Gibcus, Johan H   +3 more
openaire   +3 more sources

3PD: Rapid design of optimal primers for chromosome conformation capture assays

BMC Genomics, 2009
Background Higher eukaryotes control the expression of their genes by mechanisms that we are just beginning to understand. A complex layer of control is the dynamic spatial organization of the nucleus.
Dieterich Christoph, Fröhler Sebastian
doaj   +1 more source

X chromosome inactivation during Drosophila spermatogenesis [PDF]

, 2007
Genes with male- and testis-enriched expression are under-represented on the Drosophila melanogaster X chromosome. There is also an excess of retrotransposed genes, many of which are expressed in testis, that have “escaped” the X chromosome and moved to ...
Baines, J., Hense, Winfried, Parsch, John, John Parsch, Baines, John F., Parsch John, Hense Winfried, Baines John F, Hense, W., John F Baines, Winfried Hense, Parsch, J.   +11 more
core   +2 more sources

Additional insights into the organization of transcriptional regulatory modules based on a 3D model of the Saccharomyces cerevisiae genome

BMC Research Notes, 2022
Objectives Transcriptional regulatory modules are usually modelled via a network, in which nodes correspond to genes and edges correspond to regulatory associations between them.
Thibault Poinsignon, Mélina Gallopin, Jean-Michel Camadro, Pierre Poulain, Gaëlle Lelandais   +4 more
doaj   +1 more source

Chromosome Conformation Capture (3C) in Budding Yeast [PDF]

Cold Spring Harbor Protocols, 2015
Chromosome conformation capture (3C) is a method for studying chromosomal organization that takes advantage of formaldehyde cross-linking to measure the spatial association of two pieces of chromatin. The 3C method begins with whole-cell formaldehyde fixation of chromatin.
Belton, Jon-Matthew, Dekker, Job
openaire   +3 more sources

Carbohydrate- and conformation-dependent cargo capture for ER-exit [PDF]

, 2005
Some secretory proteins leave the endoplasmic reticulum (ER) by a receptor-mediated cargo capture mechanism, but the signals required for the cargo-receptor interaction are largely unknown.
Appenzeller-Herzog, C., Lopez-Otin, C., Santamaria, I., Nyfeler, B., Burkhard, P., Hauri, H. -P.   +5 more
core   +1 more source

Hypergraph Analysis Toolbox for Chromosome Conformation [PDF]

, 2022
Recent advances in biological technologies, such as multi-way chromosome conformation capture (3C), require development of methods for analysis of multi-way interactions.
Indika Rajapakse, Stansbury, Cooper, Chen, Can, Rajapakse, Indika, Salman, Rahmy, Amit Surana, Cooper Stansbury, Joshua Pickard, Pickard, Joshua, Bloch, Anthony, Sion Kim, Kim, Sion, Surana, Amit, Anthony Bloch, Can Chen, Rahmy Salman   +15 more
core   +1 more source

Simultaneous Profiling of Chromosome Conformation and Gene Expression in Single Cells

Bio-Protocol, 2023
Rapid development in single-cell chromosome conformation capture technologies has provided valuable insights into the importance of spatial genome architecture for gene regulation.
Yujie Chen, Heming Xu, Zhiyuan Liu, Dong Xing   +3 more
doaj   +1 more source

Primer sequences for Chromosome Conformation capture Assay.

, 2012
Primer sequences for Chromosome Conformation capture Assay.
Jae-Seon So (151210), Arijita Jash (151189), Sin-Hyeog Im (109600), Kangsun Yun (151197), Anupama Sahoo (151204)   +4 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source