Results 61 to 70 of about 1,497,962 (254)

Microfluidics-Based Chromosome Conformation Capture (3C) Technology for Examining Chromatin Organization with a Low Quantity of Cells. [PDF]

Anal Chem, 2018
Detecting three-dimensional (3D) genome organization in the form of physical interactions between various genomic loci is of great importance for understanding transcriptional regulations and cellular fate.
Sun C, Lu C.
europepmc   +2 more sources

Capturing chromosome conformation in Crenarchaea

Molecular Microbiology
AbstractWhile there is a considerable body of knowledge regarding the molecular and structural biology and biochemistry of archaeal information processing machineries, far less is known about the nature of the substrate for these machineries—the archaeal nucleoid.
Elyza Pilatowski‐Herzing, Rachel Y. Samson, Naomichi Takemata, Catherine Badel, Peter B. Bohall, Stephen D. Bell   +5 more
openaire   +2 more sources

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast

, 2015
Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library.
Dekker, Job, Belton, Jon-Matthew
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

SnapShot: Chromosome Conformation Capture

Cell, 2012
Coverage Commonly < 1Mb Commonly < 1Mb Genome-wide Genome-wide Commonly < 1Mb Genome-wideDetection Locus-specifi c PCR HT-sequencing HT-sequencing HT-sequencing Locus-specifi c qPCR HT-sequencingLimitations Low throughput and coverageLimited coverage Limited to one viewpointRely on one chromatin-associated factor, disregarding other contactsExamples ...
Hakim, Ofir, Misteli, Tom
openaire   +2 more sources

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Computational methods for analyzing genome-wide chromosome conformation capture data

, 2018
In all organisms, chromatin is packed to fulfil structural constraints and functional requirements. The hierarchical model of chromatin organization in the 3D nuclear space encompasses different topologies at diverse scale lengths, with chromosomes ...
Nicoletti, Chiara, Silvio Bicciato, Forcato, Mattia, Chiara Nicoletti, Bicciato, Silvio, Mattia Forcato   +5 more
core   +1 more source

Cut-C: cleavage under tethered nuclease for conformational capture

BMC Genomics, 2019
Background Deciphering the 3D structure of the genome is essential for elucidating the regulatory mechanisms of gene expression in detail. Existing methods, such as chromosome conformation capture (3C) and Hi-C have enabled the identification of novel ...
Takashi Shimbo, Machika Kawamura, Edward Wijaya, Eiichi Takaki, Yasufumi Kaneda, Katsuto Tamai   +5 more
doaj   +1 more source

Deregulation of KSHV latency conformation by ER-stress and caspase-dependent RAD21-cleavage. [PDF]

PLoS Pathogens, 2017
Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) is a human gammaherpesvirus recognized as the principal causative agent of KS and primary effusion lymphoma (PEL).
Alessandra De Leo, Horng-Shen Chen, Chih-Chi Andrew Hu, Paul M Lieberman   +3 more
doaj   +1 more source