Results 61 to 70 of about 1,560,009 (266)

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Capturing chromosome conformation in Crenarchaea

Molecular Microbiology
AbstractWhile there is a considerable body of knowledge regarding the molecular and structural biology and biochemistry of archaeal information processing machineries, far less is known about the nature of the substrate for these machineries—the archaeal nucleoid.
Elyza Pilatowski‐Herzing, Rachel Y. Samson, Naomichi Takemata, Catherine Badel, Peter B. Bohall, Stephen D. Bell   +5 more
openaire   +2 more sources

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Exploiting native forces to capture chromosome conformation in mammalian cell nuclei

Molecular Systems Biology, 2016
Mammalian interphase chromosomes fold into a multitude of loops to fit the confines of cell nuclei, and looping is tightly linked to regulated function. Chromosome conformation capture (3C) technology has significantly advanced our understanding of this ...
Lilija Brant, Theodore Georgomanolis, Milos Nikolic, Chris A Brackley, Petros Kolovos, Wilfred van Ijcken, Frank G Grosveld, Davide Marenduzzo, Argyris Papantonis   +8 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

SnapShot: Chromosome Conformation Capture

Cell, 2012
Coverage Commonly < 1Mb Commonly < 1Mb Genome-wide Genome-wide Commonly < 1Mb Genome-wideDetection Locus-specifi c PCR HT-sequencing HT-sequencing HT-sequencing Locus-specifi c qPCR HT-sequencingLimitations Low throughput and coverageLimited coverage Limited to one viewpointRely on one chromatin-associated factor, disregarding other contactsExamples ...
Hakim, Ofir, Misteli, Tom
openaire   +2 more sources

Cut-C: cleavage under tethered nuclease for conformational capture

BMC Genomics, 2019
Background Deciphering the 3D structure of the genome is essential for elucidating the regulatory mechanisms of gene expression in detail. Existing methods, such as chromosome conformation capture (3C) and Hi-C have enabled the identification of novel ...
Takashi Shimbo, Machika Kawamura, Edward Wijaya, Eiichi Takaki, Yasufumi Kaneda, Katsuto Tamai   +5 more
doaj   +1 more source

Deregulation of KSHV latency conformation by ER-stress and caspase-dependent RAD21-cleavage. [PDF]

PLoS Pathogens, 2017
Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) is a human gammaherpesvirus recognized as the principal causative agent of KS and primary effusion lymphoma (PEL).
Alessandra De Leo, Horng-Shen Chen, Chih-Chi Andrew Hu, Paul M Lieberman   +3 more
doaj   +1 more source

GenomeDISCO: a concordance score for chromosome conformation capture experiments using random walks on contact map graphs. [PDF]

Bioinformatics, 2018
Motivation The three-dimensional organization of chromatin plays a critical role in gene regulation and disease. High-throughput chromosome conformation capture experiments such as Hi-C are used to obtain genome-wide maps of three ...
Ursu O, Boley N, Taranova M, Wang YXR, Yardimci GG, Stafford Noble W, Kundaje A.   +6 more
europepmc   +2 more sources

Spatial and single‐nuclei transcriptomics reveals idiosyncratic and generic patterns in papillary and anaplastic thyroid cancers

Molecular Oncology, EarlyView.
Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur, Joel Rodrigues Vitória, Manuel Saiselet, Ligia Craciun, Denis Larsimont, Anne Lefort, Frédérick Libert, Carine Maenhaut, Sabine Costagliola, Maxime Tarabichi, Mirian Romitti, Vincent Detours   +11 more
wiley   +1 more source