Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens [PDF]
, 2012 BACKGROUND: The aim of this study was to optimize quantitative (real-time) polymerase chain reaction (qPCR) assays for 8 major periodontal pathogens, i.e.
Cosyn, Jan +8 more
core +1 more source
Chromosome segregation impacts on cell growth and division site selection in Corynebacterium glutamicum. [PDF]
, 2013 Spatial and temporal regulation of bacterial cell division is imperative for the production of viable offspring. In many rod-shaped bacteria, regulatory systems such as the Min system and nucleoid occlusion ensure the high fidelity of midcell divisome ...
Bramkamp, Marc +3 more
core +7 more sources
Mechanisms for Chromosome Segregation in Bacteria
Frontiers in Microbiology, 2021 The process of DNA segregation, the redistribution of newly replicated genomic material to daughter cells, is a crucial step in the life cycle of all living systems.
Christos Gogou +2 more
doaj +1 more source
Spatial organization of bacterial chromosomes [PDF]
Current Opinion in Microbiology, 2014 Bacterial chromosomes are organized in stereotypical patterns that are faithfully and robustly regenerated in daughter cells. Two distinct spatial patterns were described almost a decade ago in our most tractable model organisms. In recent years, analysis of chromosome organization in a larger and more diverse set of bacteria and a deeper ...
Xindan, Wang, David Z, Rudner
openaire +2 more sources
Optically mapping multiple bacterial genomes simultaneously in a single run. [PDF]
PLoS ONE, 2011 Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this ...
Matthew C Riley +3 more
doaj +1 more source
Rapid mapping of chromosomal breakpoints: from blood to BAC in 20 days [PDF]
, 2009 Structural chromosome aberrations and associated segmental or chromosomal aneusomies are major causes of reproductive failure in humans. Despite the fact that carriers of reciprocal balanced translocation often have no other clinical symptoms or ...
Baumgartner, Adolf +7 more
core +2 more sources
Bacterial artificial chromosomes improve recombinant protein production in mammalian cells
BMC Biotechnology, 2009 Background The development of appropriate expression vectors for large scale protein production constitutes a critical step in recombinant protein production.
Bauer Anton +4 more
doaj +1 more source
Structure of metaphase chromosomes: a role for effects of macromolecular crowding. [PDF]
PLoS ONE, 2012 In metaphase chromosomes, chromatin is compacted to a concentration of several hundred mg/ml by mechanisms which remain elusive. Effects mediated by the ionic environment are considered most frequently because mono- and di-valent cations cause ...
Ronald Hancock
doaj +1 more source
Bacterial chromosome extraction and isolation [PDF]
Lab on a Chip, 2002 We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.
Christelle, Prinz +4 more
openaire +2 more sources
Double-strand break repair and homologous recombination in Schizosaccharomyces pombe [PDF]
, 2006 In recent years our understanding of double strand break repair and homologous recombination in Schizosaccharomyces pombe has increased significantly, and the identification of novel pathways and genes with homologues in higher eukaryotes has increased ...
Ahmad +102 more
core +2 more sources