Differences in the localization and morphology of chromosomes in the human nucleus [PDF]
Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19.
Perry, P +5 more
core +7 more sources
Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes [PDF]
Copyright: © 2010 Stimpson et al.Genome rearrangement often produces chromosomes with two centromeres (dicentrics) that are inherently unstable because of bridge formation and breakage during cell division. However, mammalian dicentrics, and particularly
Sullivan, Beth A. +35 more
core +2 more sources
Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers [PDF]
Specific amplification of human sequences of up to several kb length has recently been accomplished in man-hamster and man-mouse somatic hybrid cell DNA by IRS-PCR (interspersed repetitive sequence — polymerase chain reaction).
Riethman, Harold C. +2 more
core +1 more source
Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries [PDF]
We established chromosomal homologies between all chromosomes of the human karyotype and that of an old world monkey (Macaca fuscata) by chromosomal in situ suppression (CISS) hybridization with human chromosome specific DNA libraries.
Stanyon, Roscoe +3 more
core +1 more source
Karyotyping human chromosomes by optical and X-ray ptychography methods [PDF]
Sorting and identifying chromosomes, a process known as karyotyping, is widely used to detect changes in chromosome shapes and gene positions. In a karyotype the chromosomes are identified by their size and therefore this process can be performed by ...
Xiong, G +18 more
core +1 more source
Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes [PDF]
Studies of higher-order chromatin arrangements are an essential part of ongoing attempts to explore changes in epigenome structure and their functional implications during development and cell differentiation.
Kreth Gregor +54 more
core +1 more source
Sorting of chromosomes by magnetic separation [PDF]
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
Dudin, Gertrud +7 more
core +1 more source
Independent stratum formation on the avian sex chromosomes reveals inter-chromosomal gene conversion and predominance of purifying selection on the w chromosome [PDF]
We used a comparative approach spanning three species and 90 million years to study the evolutionary history of the avian sex chromosomes. Using whole transcriptomes, we assembled the largest cross-species dataset of W-linked coding content to date.
Wright, Alison E +9 more
core +1 more source
Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]
Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Thomas +9 more
core +1 more source
Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones [PDF]
Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (
Laner, A. +4 more
core +1 more source

