Multicolour interphase cytogenetics: 24 chromosome probes, 6 colours, 4 layers [PDF]
From the late 1980s onwards, the use of DNA probes to visualise sequences on individual chromosomes (fluorescent in-situ hybridisation - FISH) revolutionised the study of cytogenetics.
Ellis, Michael +9 more
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Stretching the Rules: Monocentric Chromosomes with Multiple Centromere Domains [PDF]
The centromere is a functional chromosome domain that is essential for faithful chromosome segregation during cell division and that can be reliably identified by the presence of the centromere-specific histone H3 variant CenH3.
Elizabeth Schroeder-Reiter +35 more
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Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries [PDF]
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including ...
Cremer, Thomas +9 more
core +1 more source
Thermodynamic pathways to genome spatial organization in the cell nucleus [PDF]
The architecture of the eukaryotic genome is characterized by a high degree of spatial organization. Chromosomes occupy preferred territories correlated to their state of activity and, yet, displace their genes to interact with remote sites in complex ...
A. Prisco +2 more
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Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Thomas Ried +23 more
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Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets [PDF]
Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes.
Cremer, Thomas, Popp, Susanne
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Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts [PDF]
This article has been made available through the Brunel Open Access Publishing Fund.Background: Radial chromosome positioning in interphase nuclei is nonrandom and can alter according to developmental, differentiation, proliferation, or disease status.
Ishita S Mehta +7 more
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Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery [PDF]
BACKGROUND: Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins ...
Vassiliki Lazou +23 more
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Modeling meiotic chromosomes indicates a size dependent contribution of telomere clustering and chromosome rigidity to homologue juxtaposition [PDF]
Meiosis is the cell division that halves the genetic component of diploid cells to form gametes or spores. To achieve this, meiotic cells undergo a radical spatial reorganisation of chromosomes.
Penfold, Christopher A. +19 more
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Evolutionary strata on the X chromosomes of the dioecious plant Silene latifolia: Evidence from new sex-linked genes [PDF]
Despite its recent evolutionary origin, the sex chromosome system of the plant Silene latifolia shows signs of progressive suppression of recombination having created evolutionary strata of different X-Y divergence on sex chromosomes. However, even after
Bergero, Roberta +3 more
core +1 more source

