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Chromosome Painting for Plant Biotechnology
2010Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress.
Akio, Kato +7 more
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C-Banding of Plant Chromosomes
2023C-banding visualizes regions of chromosomes containing constitutive heterochromatin. It creates distinct patterns along the chromosome length and allows precise chromosome identification if C-bands are present in sufficient numbers. It is performed on chromosome spreads generated from fixed material, usually root tips or anthers.
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Uptake of isolated plant chromosomes by plant protoplasts
Planta, 1981For mass isolation of plant metaphase chromosomes, cultured cells of wheat (Triticum monococcum) and parsley (Petroselinum hortense) were synchronized by hydroxyurea and colchicine treatment. This synchronization procedure resulted in high mitotic synchrony, especially in suspension cultures of parsley in which 80% of the cells were found to be at the ...
L, Szabados, G, Hadlaczky, D, Dudits
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Chromosome organization and dynamics in plants
Current Opinion in Plant Biology, 2010The past few years have brought renewed interest in understanding the dynamics of chromosomes in interphase cells as well as during cell division, particularly meiosis. This research has been fueled by new imaging methods, particularly three-dimensional, high-resolution, and live microscopy.
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C-Banding of Plant Chromosomes
2016C-banding is used to differentially stain metaphase chromosomes in organisms having appreciable amounts of constitutive heterochromatin. Its primary benefits are that it is an inexpensive and a relatively fast method of identifying individual chromosomes and morphological or karyotypic variation, including large chromosomal rearrangements and ...
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Engineering chromosome number in plants
ScienceChromosome engineering produces a reduced eight-chromosome karyotype in Arabidopsis thaliana
Feng, Zhang, R Kelly, Dawe
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Plant hormone regulation of abiotic stress responses
Nature Reviews Molecular Cell Biology, 2022Rainer Waadt +2 more
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Climate change impacts on plant pathogens, food security and paths forward
Nature Reviews Microbiology, 2023Brajesh K Singh +2 more
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Evasion of plant immunity by microbial pathogens
Nature Reviews Microbiology, 2022Yuanchao Wang +2 more
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