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Engineering of plant chromosomes

Chromosome Research, 2015
Engineered minimal chromosomes with sufficient mitotic and meiotic stability have an enormous potential as vectors for stacking multiple genes required for complex traits in plant biotechnology. Proof of principle for essential steps in chromosome engineering such as truncation of chromosomes by T-DNA-mediated telomere seeding and de novo formation of ...
Michael Florian, Mette, Andreas, Houben
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Plant B Chromosomes

2010
B chromosomes are dispensable elements of the genome that do not recombine with the A chromosomes of the regular complement and that follow their own evolutionary pathway. Here, we survey current knowledge on the DNA/chromatin composition, origin, and drive mechanisms of B chromosomes and discuss the potential research applications of supernumerary ...
Andreas, Houben   +2 more
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Chromosome painting in plants

Methods in Cell Science, 2001
The current 'state-of-art' as to chromosome painting in plants is reviewed. We define different situations described as painting so far: i) Genomic in situ hybridisation (GISH) with total genomic DNA to distinguish alien chromosomes on the basis of divergent dispersed repeats, ii) 'Chromosomal in situ suppression' (CISS) hybridisation with chromosome ...
Schubert, I.   +3 more
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Plant Sex Chromosomes

Annual Review of Plant Biology, 2008
Although individuals in most flowering plant species, and in many haploid plants, have both sex functions, dioecious species—in which individuals have either male or female functions only—are scattered across many taxonomic groups, and many species have genetic sex determination.
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Plant Chromosomes

2019
Chromosome research in biotechnology, biodiversity and genetics chromosome analysis - scopes and advances molecular pattern analysis and gene mapping in chromosomes chromosome manipulation and engineering - genome and gene transfer microdissection and chromosome engineering.
Arun Kumar Sharma, Archana Sharma
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Sorting of plant chromosomes

2001
Publisher Summary This chapter provides an overview of plant flow cytogenetics. The high-yield procedure for preparation of chromosome suspensions based on the use of root meristems is relatively simple and can be modified to different plant species. The use of hairy root cultures represents an elegant solution for those genotypes that are difficult ...
J, Dolezel   +5 more
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Microdissection and chromosome painting of plant B chromosomes

Methods in Cell Science, 2001
Plant chromosome microdissection techniques together with different isolation and amplification methods of microisolated DNA are described. Such isolated DNA was used to 'chromosome paint' B chromosomes of the dicot Brachycome dichromosomatica and the monocot Secale cereale. It is demonstrated that the specific painting of the described chromosomes was
A, Houen, B L, Field, V A, Saunders
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Chromosomal imprinting in plants

Current Opinion in Genetics & Development, 1998
Chromosomal imprints in the broadest sense can arise in somatic as well as germline cells. They can be imposed through the modification of chromosomal proteins or by the modification of chromosomal DNA, and they typically effect the expression of nearby genes.
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PRINS on Plant Chromosomes

2006
The introduction of primed in situ labeling (PRINS) into plant cytogenetics provided a novel means for fast and highly specific visualization of DNA sequences on chromosomes and in interphase nuclei. Although the technique does not reach the sensitivity of fluorescence in situ hybridization that is needed for detection of single-copy targets, it is ...
Jirí, Macas   +3 more
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Sex chromosomes in flowering plants

American Journal of Botany, 2007
Sex chromosomes in dioecious and polygamous plants evolved as a mechanism for ensuring outcrossing to increase genetic variation in the offspring. Sex specificity has evolved in 75% of plant families by male sterile or female sterile mutations, but well‐defined heteromorphic sex chromosomes are known in only four plant families.
Ray, Ming   +3 more
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