Computational prediction and experimental validation of
Background This study reports the first collection of validated microRNA genes in the sea squirt, Ciona intestinalis. MicroRNAs are processed from hairpin precursors to ~22 nucleotide RNAs that base pair to target mRNAs and inhibit expression.
Pasquinelli Amy E +3 more
doaj +1 more source
Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination [PDF]
Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C.
Thia, Joshua +6 more
core +1 more source
Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics. [PDF]
We previously reported the discovery of a fluorescent protein voltage probe, ArcLight, and its derivatives that exhibit large changes in fluorescence intensity in response to changes of plasma membrane voltage.
Zhou Han +5 more
doaj +1 more source
ANP (Atrial Natriuretic Peptide) presence in the heart of a tunicate, Ciona intestinalis.
Atrial natriuretic peptide was found in the heart of vertebrates, we studied the ANP presence in the heart of Ciona intestinalis. This is animal is very important because of the its evolutionary position between invertebrates and vertebrates.
Aldo Gerbino +3 more
doaj +1 more source
Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis [PDF]
Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines, enhancer/gene traps and insertional mutagenesis.
SASAKURA Yasunori, Yasunori Sasakura
core +2 more sources
Background Computational methods for identifying selenoproteins have been developed rapidly in recent years. However, it is still difficult to identify the open reading frame (ORF) of eukaryotic selenoprotein gene, because the TGA codon for a ...
Jiang Liang, Liu Qiong, Ni Jiazuan
doaj +1 more source
Tissue/organ-specific genes (TSGs) are important not only for understanding organ development and function, but also for investigating the evolutionary lineages of organs in animals.
Shin Matsubara +4 more
doaj +1 more source
MiniSOG2-mediated Specific Photoablation of Motor Neurons in Ascidian Embryos
When understanding the neuronal function of a specific neural circuit, single-cell level photoablation of a targeted cell is one of the useful experimental approaches.
Madoka Utsumi +3 more
doaj +1 more source
Gene expression and regulation of molecules involved in pharynx inflammatory response induced by LPS in Ciona intestinalis [PDF]
In the ascidian Ciona intestinalis, the pharynx (hemopoietic organ) connects the external environment to the gastrointestinal system for two main activities, respiration and food collection, potentially exposing the ascidian to high concentrations of ...
A Vizzini
doaj +2 more sources
Refining the Ciona intestinalis model of central nervous system regeneration. [PDF]
BACKGROUND: New, practical models of central nervous system regeneration are required and should provide molecular tools and resources. We focus here on the tunicate Ciona intestinalis, which has the capacity to regenerate nerves and a complete adult ...
Carl Dahlberg +5 more
doaj +1 more source

