Results 121 to 130 of about 704,101 (306)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

A unified time series classification framework via adaptive Gaussian image representation

open access: yesScientific Reports
Real-world time series classification (TSC) is frequently hindered by significant data heterogeneity, characterized by complex multivariate dependencies and variable sequence lengths.
Xinyue Ren   +3 more
doaj   +1 more source

A virtual visualization method for improving the manufacturing accuracy based VPP 3D printers

open access: yesHeliyon
Compared to traditional vat photopolymerization 3D printing methods, pixel blending technique provides greater freedom in terms of user-defined lighting sources.
Zhengdong Yu   +7 more
doaj   +1 more source

Exploring Circle Packing Algorithms.

open access: yes, 2016
We present an interactive tool for visualizing and experimenting with different circle packing algorithms.
Kevin Pratt   +2 more
openaire   +3 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

open access: yesFEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru   +6 more
wiley   +1 more source

A Two-Stage Bin Packing Algorithm for Minimizing Machines and Operators in Cyclic Production Systems

open access: yesAlgorithms
This study presents a novel, two-stage algorithm that minimizes the number of machines and operators required to produce multiple product types repeatedly in cyclic scheduling. Our algorithm treats the problem of minimum machines as a bin packing problem
Yossi Hadad, Baruch Keren
doaj   +1 more source

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

open access: yesFEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Pilot Production of High Strength Low Alloy Steel Q345B Supper-Heavy Plate by Compound-Rolling Process

open access: yesTeshugang, 2014
The compound-slab (/mm) 415 x 1 630 x2 570 for compound-rolling is got by removing the scale of awaiting compound-rolling faces of double (/mm)210 x 1 630 x 2 570 Q345B steel casting slabs, cutting circle welding V and welding together. By vacuum-pumping
陈振业   +5 more
doaj  

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