Results 121 to 130 of about 251,975 (292)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Investigating helix-helix interactions in the transmembrane domains of membrane proteins [PDF]

Helix-helix interactions between membrane-spanning transmembrane (TM) domains have been shown to drive the assembly of α-helical membrane proteins within biological membranes.
King, Gavin W.
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Investigation of possible driving forces on counter-intuitive thermal collapse of intrinsically disordered proteins

, 2016
"Intrinsically disordered proteins are an emerging class of proteins that contain no secondary or tertiary structure. Through bioinformatics studies it has been predicted that a third of the human proteome consists of proteins containing intrinsic ...
Cormier, Zachary, NC DOCKS at East Carolina University   +1 more
core  

Raman optical activity demonstrates poly(L-proline) II helix in the N-terminal region of the ovine prion protein: Implications for function and misfunction

, 2004
The aqueous solution structure of the full-length recombinant ovine prion protein PrP25-233, together with that of the N-terminal truncated version PrP94-233, have been studied using vibrational Raman optical activity (ROA) and ultraviolet circular ...
Rhie, A G O, Nielsen, Kurt, Blanch, EW, Rhie, AGO, Gill, AC, Hope, James, Hecht, L, Gill, Andrew C., Gill, A C, Blanch, E W, Barron, Laurence D., Barron, L D, Nielsen, K, Hope, J, Hecht, Lutz, Barron, LD, Blanch, Ewan W., Rhie, Alexandre G. O., Rhie, Alexandre G O   +18 more
core   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Discovering the bacterial circular proteins

The Journal of Biological Chemistry, 2012
Over recent years, several examples of natural ribosomally synthesized circular proteins and peptides from diverse organisms have been described. They are a group of proteins for which the precursors must be post-translationally modified to join the N and C termini with a peptide bond.
Montalban-Lopez, Manuel, Sanchez-Hidalgo, Marina, Cebrian, Ruben, Maqueda, Mercedes   +3 more
openaire   +1 more source

Identification and Structural Characterisation of Novel Outer Membrane Proteins in the genus Borrelia

Although considered Gram-negative the outer membrane (OM) of Borrelia is unique, consisting of a variety of glycolipids, a significant number of surface exposed lipoproteins and a smaller number of integral membrane-spanning β-barrels.
Dyer, Adam
core  

Targeting C-terminal binding proteins (CtBPs) using genetic selection

, 2011
There are many protein-protein interactions that are vital for cellular processes such as signal transduction, structural organisation and apoptosis. In this study we decipher the role of the protein-protein interaction of C terminal Binding Proteins ...
Nijjar, Sharandip K.
core