Results 51 to 60 of about 678,144 (288)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin

Gut Pathogens, 2020
Background Helicobacter pylori bacterium is a major cause of gastritis. With increasing use of antibiotics to treat infections, mutation resistant strains have emerged in most human populations.
Yangxia Li, Tangshan Lv, Chaochao He, Hongyan Wang, David S. Cram, Linfu Zhou, Jun Zhang, Weiqin Jiang   +7 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

rds: mucopolysaccharidoses; glycosaminoglycans; clinical laboratory techniques; biological markers

Paediatria Croatica, 2013
U rastućem broju nasljednih metaboličkih bolesti lizosomske bolesti nakupljanja čine relativno malu skupinu od 60-tak bolesti. Njihova podskupina, mukopolisaharidoze (MPS), obuhvaća samo sedam tipova bolesti (uz podtipove), koje su zbog patofi ziološkekompleksnosti, progresivnog tijeka, multiorganske zahvaćenosti te izrazite kliničke heterogenosti i ...
Ksenija Fumić, Karmen Bilić, Marija Zekušić, Ana Škaričić, Ivo Barić   +4 more
openaire   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Use and positivity of laboratory tests at Las Tunas provincial hospital

Revista Electrónica Dr. Zoilo E. Marinello Vidaurreta, 2017
Background: the laboratories must offer the tests to aid doctors in their clinical practice, providing them with the information for the diagnosis, prevention or treatment of diseases.Objective: to describe the use of tests which have been at the doctors’
Jenny de la Caridad Hernández Betancourt, Ariel Segura Fuentes, Jorge Enrique Reyes Membrado, María Mercedes Bello Rodríguez, Bárbara Faustina Martínez Martínez   +4 more
doaj  

Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum

Frontiers in Medicine, 2018
Viscous sputum specimens usually cannot undergo automated extraction, and thus, a pre-homogenization process is desirable before isolating nucleic acids for real-time reverse transcription PCR.
Fei Yu, Ting Qiu, Ying Zeng, Yiyin Wang, Shufa Zheng, Xiao Chen, Yu Chen   +6 more
doaj   +1 more source

COVID-19: laboratory diagnosis for clinicians. An updating article

São Paulo Medical Journal, 2020
COVID-19 (coronavirus disease 2019) is an infectious disease caused by the new coronavirus associated with severe acute respiratory syndrome 2 (SARS-CoV-2).
Luisane Maria Falci Vieira, Eduardo Emery, Adagmar Andriolo   +2 more
doaj   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source