Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Statement to the Clinical Laboratory Improvements Advisory Committee on the CLIA Regulations Assessment Workgroup [PDF]
The College of American Pathologists (CAP) appreciates the opportunity to provide written comments to the Clinical Laboratory Improvement Advisory Committee (CLIAC) on the Clinical Laboratory Improvements Amendments of 1988 (CLIA) Regulations Assessment ...
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Antimicrobial resistance in the practice of primary care physicians: infection diagnosis
Family Medicine & Primary Care ReviewThe selection of appropriate specimens for diagnostic testing constitutes a critical step in the treatment process, as the data thus generated forms the foundation for evidence-based medical decisions.
Hanna Laudy-Wiaderny, Aneta Nitsch-Osuch
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Clinical laboratory COVID-19 response call : March 22, 2021 [PDF]
CDC\u2019s Division of Laboratory Systems (DLS) convenes regular calls with clinical laboratories to discuss the nation\u2019s clinical laboratory response to coronavirus disease (COVID-19).
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Clinical laboratory COVID-19 response call : October 19, 2020 [PDF]
CDC\u2019s Division of Laboratory Systems (DLS) convenes regular calls with clinical laboratories to discuss the nation\u2019s clinical laboratory response to coronavirus disease (COVID-19).
core +4 more sources
Routine laboratory data for predicting 30-day emergency department revisits: the AXIS-2 risk score
BMC Emergency MedicineBackground Thirty-day emergency department (ED) revisit is a major quality indicator reflecting morbidity and healthcare burden. Laboratory data obtained during outpatient encounters may capture underlying biological stress axes.
Ferhat Demirci, Aylin Demirci
doaj +1 more source
A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma
FEBS Letters, EarlyView.Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos +9 more
wiley +1 more source
Clinical laboratory COVID-19 response call : April 5, 2021 [PDF]
CDC\u2019s Division of Laboratory Systems (DLS) convenes regular calls with clinical laboratories to discuss the nation\u2019s clinical laboratory response to coronavirus disease (COVID-19).
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Frontiers in Medicine, 2018 Viscous sputum specimens usually cannot undergo automated extraction, and thus, a pre-homogenization process is desirable before isolating nucleic acids for real-time reverse transcription PCR.
Fei Yu +6 more
doaj +1 more source