Results 51 to 60 of about 734,729 (308)

Use and positivity of laboratory tests at Las Tunas provincial hospital

Revista Electrónica Dr. Zoilo E. Marinello Vidaurreta, 2017
Background: the laboratories must offer the tests to aid doctors in their clinical practice, providing them with the information for the diagnosis, prevention or treatment of diseases.Objective: to describe the use of tests which have been at the doctors’
Jenny de la Caridad Hernández Betancourt, Ariel Segura Fuentes, Jorge Enrique Reyes Membrado, María Mercedes Bello Rodríguez, Bárbara Faustina Martínez Martínez   +4 more
doaj  

Prevalence and genetic diversity of human diarrheagenic Escherichia coli isolates by multilocus sequence typing

International Journal of Infectious Diseases, 2018
Background: The population structure of human diarrheagenic Escherichia coli (DEC) isolates derived from worldwide collections remains undefined. Methods: A total of 1196 clinical isolates were obtained from a multilocus sequence typing (MLST) database ...
Fei Yu, Xiao Chen, Shufa Zheng, Dongsheng Han, Yiyin Wang, Ruonan Wang, Baohong Wang, Yu Chen   +7 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Clinical risk factors associated with multidrug-resistant tuberculosis (MDR-TB) in Mali

International Journal of Infectious Diseases, 2019
Background: MDR-TB is a major threat to global TB control. In 2015, 580,000 were treated for MDR-TB worldwide. The worldwide roll-out of GeneXpert MTB/RIF® has improved diagnosis of MDR-TB; however, in many countries laboratories are unable to assess ...
Bocar Baya, Chad J. Achenbach, Bourahima Kone, Yacouba Toloba, Djeneba K. Dabitao, Bassirou Diarra, Drissa Goita, Seydou Diabaté, Mamoudou Maiga, Dianguina Soumare, Khadidia Ouattara, Tenin Kanoute, Gaoussou Berthe, Youssouf M. Kamia, Yeya dit Sadio Sarro, Moumine Sanogo, Antieme C.G. Togo, Bindongo P.P. Dembele, Nadie Coulibaly, Amadou Kone, Maxwell Akanbi, Michael Belson, Sounkalo Dao, Susan Orsega, Sophia Siddiqui, Seydou Doumbia, Robert L. Murphy, Souleymane Diallo   +27 more
doaj   +1 more source

COVID-19: laboratory diagnosis for clinicians. An updating article

São Paulo Medical Journal, 2020
COVID-19 (coronavirus disease 2019) is an infectious disease caused by the new coronavirus associated with severe acute respiratory syndrome 2 (SARS-CoV-2).
Luisane Maria Falci Vieira, Eduardo Emery, Adagmar Andriolo   +2 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin

Gut Pathogens, 2020
Background Helicobacter pylori bacterium is a major cause of gastritis. With increasing use of antibiotics to treat infections, mutation resistant strains have emerged in most human populations.
Yangxia Li, Tangshan Lv, Chaochao He, Hongyan Wang, David S. Cram, Linfu Zhou, Jun Zhang, Weiqin Jiang   +7 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Lupus anticoagulant testing during anticoagulation, including direct oral anticoagulants

Research and Practice in Thrombosis and Haemostasis, 2022
Background Lupus anticoagulants (LA) are one laboratory criterion for classification of antiphospholipid syndrome, with presence of vascular thrombosis and/or pregnancy/fetal morbidity being clinical criteria. The presence of LA is detected (or excluded)
Emmanuel J. Favaloro, Leonardo Pasalic
doaj   +1 more source

Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers

Molecular Oncology, EarlyView.
This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel, Peyman Zarrineh, Nathnael T. Tuffa, Jigar H. Sheth, Sumitra Mohan, Mudassar Iqbal, Sankari Nagarajan   +6 more
wiley   +1 more source