Results 81 to 90 of about 790,447 (335)

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Cloning of Canine Osteosarcoma Cells.

Journal of Veterinary Medical Science, 1995
Six clonal cells were established from a canine osteosarcoma cell line (POS) by a limiting dilution method using feeder cells. Whereas histology of mass developed in nude mice by transplantation of POS cells revealed various cell types, clonal cells were morphologically consistent in size and shape.
K, Nozaki, K, Okuyama, T, Kadosawa, H, Nakayama, N, Goto, N, Sasaki, A, Takeuchi   +6 more
openaire   +3 more sources

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Oxidative Stress and Apoptosis Contributed to Nonylphenol-Induced Cell Damage in Mouse NCTC Clone 1469 Cells

Journal of Chemistry, 2020
Nonylphenol (NP) is considered an environmental toxicant and endocrine-disrupting compound. The present study aimed to investigate the effects of NP on NCTC Clone 1469, nonparenchymal hepatocytes, and to study the molecular basis of NP-induced liver ...
Xiaozhen Liu, Yangjie Chen, Shaoping Nie, Fuxiang Li, Zhaoliang Zhu, Gaoyi Peng, Qiang Yu, Mingyong Xie   +7 more
doaj   +1 more source

High throughput sequencing in acute lymphoblastic leukemia reveals clonal architecture of central nervous system and bone marrow compartments [PDF]

, 2017
No abstract ...
Adams, Stuart, Bartram, Jack, Brooks, Tony, Edwards, Darren, Goulden, Nick, Halsey, Christina, Hubank, Mike, Inglott, Sarah, Wright, Gary, Yousafzai, Yasar   +9 more
core   +3 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Cloning and Expression of hGAD65 Gene in E. Coli BL21

Indonesian Journal of Biotechnology, 2013
The aim of this study is to construct the hGAD65 gene and to identify the hGAD65 clone by using PCR & RFLP. The samples were derived from normal person & DM patient’s blood.
Rista Nikmatu Rohmah, Soraya Widyasari, A. Aulanni’am, F. Fatchiyah   +3 more
doaj   +1 more source

Cloning of terminal transferase cDNA by antibody screening [PDF]

, 1984
A cDNA library was prepared from a terminal deoxynucleotidyltransferase-containing thymoma in the phage vector λgt11. By screening plaques with anti-terminal transferase antibody, positive clones were identified of which some had β-galactosidase-cDNA ...
Baltimore, David, Landau, Nathaniel R., Silverstone, Allen E., St. John, Thomas P., Weissman, Irving L., Wolf, Susan C.   +5 more
core  

A way to synchronize models with seismic faults for earthquake forecasting: Insights from a simple stochastic model

, 2006
Numerical models are starting to be used for determining the future behaviour of seismic faults and fault networks. Their final goal would be to forecast future large earthquakes.
Aki, Amalio F. Pacheco, Baba, Bak, Ben-Zion, Boness, Bowman, Castellaro, Dahmen, Das, Das, Dowrick, Drossel, Fitzenz, Goltz, González, González, Grant, Gutenberg, Gutenberg, Gómez, Hainzl, Hainzl, Hainzl, Harris, Hashimoto, Heimpel, Helmstetter, Henley, Hickman, Houghton, Ikeda, Ishimoto, Javier B. Gómez, Kanamori, Kanamori, Kato, Keilis-Borok, Kisslinger, Kossobokov, Kuroki, Lei, López-Ruiz, Main, Mason, Michael, Miguel Vázquez-Prada, Miller, Molchan, Moreno, Newman, Okada, Olami, Palmer, Pepke, Preston, Reasenberg, Robinson, Robinson, Rubin, Rundle, Rundle, Rundle, Rundle, Rundle, Scholz, Scholz, Schorlemmer, Schwartz, Seeber, Sieh, Soloviev, Steacy, Stirling, Stirling, Tanioka, Tiampo, Tsukahara, Turcotte, Vázquez-Prada, Vázquez-Prada, Ward, Wells, Wesnousky, Wesnousky, Wyss, Yabuki, Yagi, Yamamoto, Yeats, Youngs, Zoback, Zöller, Álvaro González   +93 more
core   +1 more source

Comprehensive Immune Monitoring of Clinical Trials to Advance Human Immunotherapy. [PDF]

, 2019
The success of immunotherapy has led to a myriad of clinical trials accompanied by efforts to gain mechanistic insight and identify predictive signatures for personalization.
Amir, El-Ad D, Babdor, Joel, Bendall, Sean C, Gherardini, Pier Federico, Hartmann, Felix J, Jones, Kyle, Krutzik, Peter, Maecker, Holden T, Marquez, Diana M, Meyer, Everett, O'Donnell, Erika, Sahaf, Bita, Sigal, Natalia, Spitzer, Matthew H   +13 more
core   +3 more sources