Results 181 to 190 of about 459,197 (264)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Prognostic significance of PNH clones in aplastic anemia treated with immunosuppression or allogeneic HSCT: a 20-year single-center experience. [PDF]

Blood Res
Haroon A, Alzahrani H, Saleh MFM, Aalahmari A, Alotaibi S, Elhassan T, Alfraih F, Alsharif F, Ahmed SO, Almohareb F, Fakih RE, Aljurf M.   +11 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Stability analysis of cocoa clones for yield in the humid tropics of India. [PDF]

Front Plant Sci
Kireeti A, Ramanandam G, Rao VG, Neeraja B, Rao NBVC, Apshara E, Sumitha S, Jerard AB.   +7 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Clonal hematopoiesis dynamics influences long-term outcomes of follicular lymphoma: Results from FIL FOLL12 trial. [PDF]

Hemasphere
Maher N, Moia R, Almasri M, Cividini L, Genuardi E, Cosentino C, Soscia R, Assanto GM, Tavarozzi R, Vegliante MC, Lorenzi L, Andorno A, Arcaini L, Ragaini S, Puccini B, Patti C, Santoro A, Margiotta-Casaluci G, Zilioli VR, Zanni M, Ronconi S, Di Raimondo F, Arcari A, Califano C, Castellino C, Conconi A, Perrone T, Mannina D, Plenteda C, Alesiani F, Rossi FG, Carella AM, Marcheselli L, Galimberti S, Ciavarella S, Bomben R, Del Giudice I, Ladetto M, Gaidano G, Luminari S, Ferrero S.   +40 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide Polymorphism. [PDF]

Bio Protoc
Mahatha AC, Ramos-Espinosa O, de Lima DS, Liu EY, Vaidyan S, Liu J.   +5 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

<i>RBX1</i> loss sensitizes tubo-ovarian, high-grade serous ovarian cells to CDK2 inhibition by SNS-032. [PDF]

Front Cell Dev Biol
Farrell AC, Lam LA, Chen H, Neudorf NM, Sajesh BV, Lepage CC, Lichtensztejn Z, McManus KJ.   +7 more
europepmc   +1 more source