Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Correction: Prp8 regulates oncogene-induced hyperplastic growth in Drosophila. [PDF]
Fernández-Espartero CH +5 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Beyond Binary: Cardiac Patterning as Probabilistic Fate Restriction. [PDF]
Ivanovitch K.
europepmc +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Improvement of the Champagne-Epernay-Geisenheim (CEG) Wine Yeast via Meiotic Spore Clone Selection. [PDF]
Muno-Bender J +8 more
europepmc +1 more source
From Antigenic Drive to Clonal Autonomy: An Update on Molecular Mechanisms of HCV-Related B-Cell Lymphomagenesis. [PDF]
Marri S +3 more
europepmc +1 more source
Neocentromeres fail to maintain DNA methylation boundaries, driving CENP-A drift, instability, and chromosome missegregation. [PDF]
Mahlke MA +16 more
europepmc +1 more source
The Drosophila ovary produces two waves of adult follicles and a previously unreported pupal wave that turns over. [PDF]
Fu WY, Spradling AC.
europepmc +1 more source
Efficient discovery of an agonistic anti-OX40 nanobody by epitope-directed approach to address enrichment-driven epitope bias. [PDF]
Iemura T +10 more
europepmc +1 more source

