This review highlights recent advances in engineering artificial antigen‐presenting cells (aAPCs) as alternatives to dendritic cells for T cell expansion. Key design principles inspired by the immunological synapse are discussed, with emphasis on strategies for polyclonal and antigen‐specific T cell expansion.
Nguyen Thi Nguyen, Yu Seok Youn
wiley +1 more source
CRISPR.BOT an autonomous platform for streamlined genetic engineering and molecular biology applications. [PDF]
Erkek F+18 more
europepmc +1 more source
Quantum Emitters in Hexagonal Boron Nitride: Principles, Engineering and Applications
Quantum emitters in hexagonal boron nitride have emerged as a promising candidate for quantum information science. This review examines the fundamentals of these quantum emitters, including their level structures, defect engineering, and their possible chemical structures.
Thi Ngoc Anh Mai+8 more
wiley +1 more source
High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles
The layer‐by‐layer (LbL) assembly of polymer films on nanoparticle (NP) surfaces is a promising technique for targeted drug delivery. Despite its success in preclinical mouse models, a current good manufacturing practices (cGMP) compliant, clinical‐scale production method has been lacking.
Ivan S. Pires+4 more
wiley +1 more source
Cloning and enhancing lumbrokinase production from local Eisenia fetida by signal peptide engineering for effective thrombosis treatment. [PDF]
Tabassum A, Zeeshan N, Afroz A.
europepmc +1 more source
A Universal Biomimetic Approach for Making Artificial Antigen‐Presenting Cells for T Cell Activation
This study shows a biomimetic silica microcapsule (SMC) fabrication method under mild conditions for making artificial antigen‐presenting cells (aAPCs). Inspired by marine biomineralization, peptide‐mediated biosilicification enables silica shell formation on emulsion templates. The resulting SMCs possess a core–shell structure, controlled fluorescence
Fei Hou+5 more
wiley +1 more source
CRISPR-BEasy: a free web-based service for designing sgRNA tiling libraries for CRISPR-dependent base editing screens. [PDF]
Chapdelaine-Trépanier V+9 more
europepmc +1 more source
Guide to Molecular Cloning Techniques [PDF]
openaire +2 more sources
A Sustainable Biotechnology Approach for Mineral Separation
Silver‑binding peptides are first identified through phage‐display screening, and then engineered into multifunctional and responsive peptides or proteins. These tailored biomolecules can selectively capture and aggregate silver particles or ions from complex mixtures, enabling rapid and efficient silver separation and recovery.
Guangze Yang+7 more
wiley +1 more source
Cloning, Expression and Functional Characterization of <i>V. vinifera</i> CAT2 Arginine Transporter. [PDF]
Pochini L+6 more
europepmc +1 more source