Results 171 to 180 of about 486,356 (308)

Full-Length cDNA Cloning, Molecular Characterization and Differential Expression Analysis of Lysophospholipase I from Ovis aries. [PDF]

Int J Mol Sci, 2016
Liu NN, Liu ZS, Hu P, Zhang Y, Lu SY, Li YS, Yang YJ, Zhang DS, Zhou Y, Ren HL.   +9 more
europepmc   +1 more source

Molecular Cloning of the Acetylcholine Receptor

Cold Spring Harbor Symposia on Quantitative Biology, 1983
J, Patrick, M, Ballivet, L, Boas, T, Claudio, J, Forrest, H, Ingraham, P, Mason, S, Stengelin, S, Ueno, S, Heinemann   +9 more
openaire   +2 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Research Progress of Laccase in Edible and Medicinal Fungi. [PDF]

J Fungi (Basel)
Zhao Y, Zhang X, Jiang Y, Song S, Han C, Dai X.   +5 more
europepmc   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement. [PDF]

Plant Genome
Gudi S, Razzaq K, Singh J, Mendoza LDR, Khan M, Gupta R, Gill U.   +6 more
europepmc   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

DNA 'Breathing' Recombination Cloning: A Mismatch-Tolerant, Temperature-Dependent Homologous Recombination Cloning Method. [PDF]

Int J Mol Sci
He Y, Ding Y, Zhang Y, Liu L, Lyu S, Fan Y.   +5 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A protocol for computational design of mRNA vaccines with high functionality and specificity. [PDF]

Biol Direct
Ramadan MA, ElGohary HM, Faraag AH, Mysara M.   +3 more
europepmc   +1 more source