Progressive Myoclonus Epilepsy: Distinctive MRI Changes in Cerebellar and Motor Networks
ABSTRACT Objective Progressive myoclonus epilepsy (PME) is a rare generalized epilepsy syndrome with a well‐characterized genetic basis. The brain networks that are affected to give rise to the distinctive symptoms of PME are less well understood. Methods Eleven individuals with PME with a confirmed genetic diagnosis and 22 controls were studied.
Jillian M. Cameron+3 more
wiley +1 more source
DAPE cloning with modified primers for producing designated lengths of 3' single-stranded ends in PCR products. [PDF]
Lee S+5 more
europepmc +1 more source
Epitope Mapping of Anti‐Neurofascin 155 Antibody in a Large Cohort of Autoimmune Nodopathy Patients
ABSTRACT Objective Autoimmune nodopathy (AN), a newly recognized disease entity, is an immune‐mediated polyneuropathy involving autoantibodies against cell adhesion molecules located in nodes of Ranvier and paranodal regions, such as neurofascin 186 (NF186) and neurofascin 155 (NF155). The present study aimed to identify the epitopes for autoantibodies
Amina A. Abdelhadi+6 more
wiley +1 more source
A suite of pre-assembled, pET28b-based Golden Gate vectors for efficient protein engineering and expression. [PDF]
Gaur D, Wohlever ML.
europepmc +1 more source
Paintig of defined chromosomal regions by in situ suppression hybridization of libraries from laser-microdissected chromosomes [PDF]
Cremer, Thomas+7 more
core +1 more source
Zhengjun Xia+12 more
semanticscholar +1 more source
Spatiotemporal Control Over Protein Release from Artificial Cells via a Light‐Activatable Protease
Stimulus‐responsive protein release is essential for intercellular communication. Mimicking this functionality in artificial cells is promising to study the working principles of cellular signaling. Herein, an engineered light‐activatable protease is implemented in a coacervate‐based artificial cell platform to establish user‐defined spatiotemporal ...
Arjan Hazegh Nikroo+4 more
wiley +1 more source
An affordable and simple method for DNA extraction from agarose suitable for downstream applications. [PDF]
Sánchez-Flores JE+3 more
europepmc +1 more source
Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells
TEV Prtoease‐mediated Releasable Actin‐binding Protein (TRAP) is a protein‐based platform consisting of a cargo tightly bound to reconstituted actin networks in synthetic cells which can be proteolyticly released from the bound actin, followed by its secretion through membrane translocation mediated by a cell‐penetrating peptide.
Mousumi Akter+3 more
wiley +1 more source
Molecular Mechanisms of Somatic Cell Cloning and Other Assisted Reproductive Technologies in Mammals: Which Determinants Have Been Unraveled Thus Far?-Current Status, Further Progress and Future Challenges. [PDF]
Samiec M.
europepmc +1 more source