Results 81 to 90 of about 759,664 (294)

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source

Bacterial diversity of symptomatic primary endodontic infection by clonal analysis

Brazilian Oral Research
The aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and ...
Letícia Maria Menezes NÓBREGA, Francisco MONTAGNER, Adriana Costa RIBEIRO, Márcia Alves Pinto MAYER, Brenda Paula Figueiredo de Almeida GOMES   +4 more
doaj   +1 more source

Sources of blood meals of sylvatic Triatoma guasayana near Zurima, Bolivia, assayed with qPCR and 12S cloning. [PDF]

PLoS Neglected Tropical Diseases, 2014
In this study we compared the utility of two molecular biology techniques, cloning of the mitochondrial 12S ribosomal RNA gene and hydrolysis probe-based qPCR, to identify blood meal sources of sylvatic Chagas disease insect vectors collected with live ...
David E Lucero, Wilma Ribera, Juan Carlos Pizarro, Carlos Plaza, Levi W Gordon, Reynaldo Peña, Leslie A Morrissey, Donna M Rizzo, Lori Stevens   +8 more
doaj   +1 more source

The structure of protein molecules : in celebration of the International Year of Crystallography, 2014 [PDF]

, 2014
Many people, including laymen, are aware of the double helical nature of the DNA molecule. A few may actually realise that it was the technique of X-ray crystallography that was the key to solving this structure.
Bonetta, Rosalin, Farrugia, Diane, Hunter, Gary J., Hunter, Therese, Vella, Marita   +4 more
core   +1 more source

The multidrug and toxin extrusion (MATE) transporter DTX51 antagonizes non‐cell‐autonomous HLS1–AMP1 signaling in a region‐specific manner

FEBS Letters, EarlyView.
The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley   +1 more source

New Clox Systems for rapid and efficient gene disruption in Candida albicans [PDF]

, 2014
Acknowledgements: We are grateful to Janet Quinn, Lila Kastora, Joanna Potrykus, Michelle Leach, and others for sharing their experiences with the Clox cassettes.
Ballou, Elizabeth R, Bohovych, Iryna, Brown, Alistair J P, Childers, Delma S, Gow, Neil A. R., Odds, Frank C, Shahana, Shahida   +6 more
core   +4 more sources

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Creating E.coli Library of Diagnostic Gene for Influenza B Virus Using Molecular Cloning Technique [PDF]

, 2014
Influenza viruses cause seasonal epidemics associated with high morbidity, especially in immunocompromised and extreme age group individuals. Many strategies for combating these viruses are in practice; however, developing and maintaining sensitive ...
Chinbat, Oyunsuvd
core  

A species-specific satellite DNA family in the genome of the coffee root-knot nematode Meloidogyne exigua : Application to molecular diagnostics of the parasite [PDF]

, 2002
A new Bg/II satellite DNA has been isolated, cloned and sequenced from the coffee root-knot nematode, Meloidogyne exigua (Nematoda: Tylenchida). It is represented as tandemly repeated sequences with a monomeric unit of 277 bp. The monomers are present at
Bongiovanni, Michel, Carneiro, Regina Maria Dechechi Gomes, Castagnone Sereno, Philippe, Randig, Onivaldo, Sarah, Jean-Louis   +4 more
core   +2 more sources

A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival

Molecular Oncology, EarlyView.
There is an unmet need in metastatic breast cancer patients to monitor therapy response in real time. In this study, we show how a noninvasive and affordable strategy based on sequencing of plasma samples with longitudinal tracking of tumour fraction paired with a statistical model provides valuable information on treatment response in advance of the ...
Emma J. Beddowes, Mario Ortega Duran, Solon Karapanagiotis, Alistair Martin, Meiling Gao, Riccardo Masina, Ramona Woitek, James Tanner, Fleur Tippin, Justine Kane, Jonathan Lay, Anja Brouwer, Stephen‐John Sammut, Suet‐Feung Chin, Davina Gale, Dana W. Y. Tsui, Sarah‐Jane Dawson, Nitzan Rosenfeld, Maurizio Callari, Oscar M. Rueda, Carlos Caldas   +20 more
wiley   +1 more source