Results 81 to 90 of about 5,041,968 (259)
Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique
JOIV: International Journal on Informatics Visualization, 2021 Bioinformatics has developed into an application tool for basic and applied research in the biomedical and biotechnology field. Polymerase Chain Reaction (PCR) is a common technique in the molecular area that has always involved bioinformatics science ...
- Mardalisa +5 more
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TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method
Frontiers in Bioengineering and Biotechnology, 2023 Molecular cloning is used in a wide variety of biological and medical research. Here, we developed a rapid and efficient DNA-assembling method for routine laboratory work.
Fang Yu +8 more
doaj +1 more source
Ion channels: structural basis for function and disease. [PDF]
, 1996 Ion channels are ubiquitous proteins that mediate nervous and muscular function, rapid transmembrane signaling events, and ionic and fluid balance. The cloning of genes encoding ion channels has led to major strides in understanding the mechanistic basis
Goldstein, SA
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Efficient Parallel Algorithm for Statistical Ion Track Simulations in Crystalline Materials
, 2008 We present an efficient parallel algorithm for statistical Molecular Dynamics simulations of ion tracks in solids. The method is based on the Rare Event Enhanced Domain following Molecular Dynamics (REED-MD) algorithm, which has been successfully applied
Beardmore +19 more
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BMC Biotechnology, 2011 Background Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. Results Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any ...
Lu Jia +5 more
doaj +1 more source
Molecular cloning of the chicken avidin cDNA [PDF]
Nucleic Acids Research, 1987 A cDNA for chicken avidin was identified in a chicken oviduct cDNA library by screening with antibodies and synthetic oligodeoxyribonucleotides. Four recombinant clones were characterized and each contained the sequence of the oligonucleotide probes used in screening.
Bert W. O'Malley +7 more
openaire +3 more sources
Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells. [PDF]
, 1998 Activation of T and natural killer (NK) cells leads to the tyrosine phosphorylation of pp36 and to its association with several signaling molecules, including phospholipase Cgamma-1 and Grb2.
Berg, SF +8 more
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Cloning, expression and purification of the general stress protein Yhbo from Escherichia coli [PDF]
Protein expression and purification. sous presse (2005) ?, 2005 We cloned, expressed and purified the Escherichia coli yhbO gene product, which is homolog to the Bacillus subtilis general stress protein 18 (the yfkM gene product), the Pyrococcus furiosus intracellular protease PfpI, and the human Parkinson disease protein DJ-1. The gene coding for YhbO was generated by amplifying the yhbO gene from E.
arxiv +1 more source
Cloning of a novel receptor expressed in rat prostate and ovary.
Proceedings of the National Academy of Sciences of the United States of America, 1996 We have cloned a novel member of the nuclear receptor superfamily. The cDNA of clone 29 was isolated from a rat prostate cDNA library and it encodes a protein of 485 amino acid residues with a calculated molecular weight of 54.2 kDa.
G. Kuiper +4 more
semanticscholar +1 more source
My career in molecular biology [PDF]
, 2002 Norman Davidson's training as a physical chemist led him to make key early contributions to the chemistry of DNA. He described the details of DNA denaturation and renaturation, concepts that still form the basis for understanding hybridization.
Davidson, Norman
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