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Cloning pigs as organ donors for humans

IEEE Engineering in Medicine and Biology Magazine, 2004
In order to overcome this shortage of organs, scientists have considered the possibility of using animals as a source of organs. Xenotransplantation, or xenografting, is the process of transplanting live organs between different species. ne choice of source animals will be of prime importance for xenotransplantation.
Liangxue, Lai, Randall S, Prather
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Cloned riboprobe for detection of a mycoplasmalike organism

Biochemical and Biophysical Research Communications, 1988
A [32P]-labeled single stranded-RNA probe (riboprobe) was constructed with plasmid vector pSP64 and used to detect and specifically identify an uncultured pathogenic mycoplasmalike organism in infected host. The riboprobe was more sensitive and reliable than complementary double stranded-DNA probe in detection of western X mycoplasmalike organism. When
I M, Lee   +3 more
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Sequence organization of cloned intracisternal a particle genes

Cell, 1980
Seven recombinant DNA clones containing mouse intracisternal A particle genes were isolated and analyzed by restriction enzyme digestion, Southern blot analysis and heteroduplex mapping. The sequence organization of the individual genes was found to differ, with one end of the gene region being most variable, while a central segment of 1.8 kb was ...
M, Ono   +3 more
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Whole-organism clone tracing using single-cell sequencing

Nature, 2018
Embryonic development is a crucial period in the life of a multicellular organism, during which limited sets of embryonic progenitors produce all cells in the adult body. Determining which fate these progenitors acquire in adult tissues requires the simultaneous measurement of clonal history and cell identity at single-cell resolution, which has been a
Alemany, Anna   +4 more
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The Organization and Evolution of Cloned Globin Genes

1980
The globin genes represent a complex set of sequences that are expressed in a coordinate fashion during the development of red blood cells. while this complex family of genes may consist of as many as ten to fourteen members [34], three of these genes have now been cloned and their entire nucleotide sequence determined. As was initially observed in the
P, Leder   +5 more
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Cloning, expression, and genomic organization of mouse mp29 gene

Biochemical and Biophysical Research Communications, 2002
Human p29 has been demonstrated in the yeast two-hybrid method and in vitro GST pull-down assay to associate with GCIP, a cyclin D interacting protein. In this study, we describe the cloning and genomic structure of the mouse homologue, mp29. The overall mouse mp29 amino acid sequence is highly identical (91%) to human p29.
Mau Sun, Chang   +5 more
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Behavior Cloning by a Self-Organizing Decision Tree

2007 IEEE International Conference on Integration Technology, 2007
It is hard to define a state space or the proper reward function in reinforcement learning to make the robot act as expected. In this paper, we demonstrate the expected behavior for a robot Then a RL-based decision tree approach which decides to split according to long-term evaluations, instead of a top-down greedy strategy which finds out the ...
Kao-Shing Hwang   +2 more
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Genetic organization and cloning of kirsten murine sarcoma virus DNA

Biochemical and Biophysical Research Communications, 1982
Summary The Kirsten murine sarcoma virus genome is a recombinant molecule derived from the Kirsten murine leukemia virus genome and sequences transduced from a normal rat cell. We show here that leukemia virus derived sequences comprise the 5′ terminal 0.5 kilobasepairs (kbp) and the 3′ terminal 1.2 kbp of the linear 6.5 kbp sarcoma virus DNA.
J D, Norton, R J, Avery
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Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2005
A cDNA encoding chymotrypsin inhibitor was constructed from the cellular RNA isolated from the venom glands of Naja atra (Taiwan cobra). The resultant amino acid sequence showed that the mature protein is comprised of 57 amino acid residues with six cysteine residues. Cloned protein was expressed and isolated from the inclusion bodies of E.
Yun-Ching, Cheng   +2 more
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Cloning, Genomic Organization, and Tissue Distribution of Human Ssf-1

Biochemical and Biophysical Research Communications, 2000
During the screening of a human placenta cDNA library, realized in order to isolate the P2Y(11) coding sequence, an unrelated cDNA was cloned. We identified a 1422 bp open reading frame encoding a human protein displaying 40% amino acid identity with the Saccharomyces cerevisiae Ssf-1, a protein involved in the second step of mRNA splicing.
Suarez Gonzalez, Nathalie   +2 more
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