Results 151 to 160 of about 630,776 (310)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Therapeutic cloning: from consequences to contradiction

, 2002
The British Parliament legalized therapeutic cloning in December 2000 despite opposition from the European Union. The watershed event in Parliament's move was the active and unprecedented government support for the generation and destruction of human ...
Coors, Marilyn E.
core  

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Autoencoding-Assisted Quantum Cloning Machine. [PDF]

Entropy (Basel)
Beh QJ, Straeter M, Sun Z, Kwek LC, Zhan Y.   +4 more
europepmc   +1 more source

HUMAN CLONING IN THE MEDIA: FROM SCIENCE FICTION TO SCIENCE PRACTICE

, 2008
List of figures -- Acknowledgements -- 1. Introduction -- 2. What is cloning? -- 3. Cloning futures -- 4. Mavericks, madmen and fallen heroes -- 5. Women's bodies in cloning discourse -- 6. Truth claims and genres -- 7.
Haran, Joan; Kitzinger, Jenny; McNeil, Maureen; and O'Riordan, Kate
core  

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Epigenetic Drift and the Generational Limit of Serial Somatic Cell Nuclear Transfer in Pigs. [PDF]

Animals (Basel)
Cheng N, Jamal MA, Li H, Li M, Xu Q, Wei HJ, Cheng W.   +6 more
europepmc   +1 more source

Cloning: On human cloning [PDF]

Nature Biotechnology, 1998
openaire   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

A high-throughput, streamlined cloning protocol to generate guide RNAs for CRISPR activation. [PDF]

Front Genome Ed
Zhu S, Tamez González AA, Alokda A, Van Raamsdonk JM.   +3 more
europepmc   +1 more source