Results 321 to 330 of about 1,949,061 (374)
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2013
Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are ...
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Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are ...
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Cloning in Plasmid Vectors: Directional Cloning
Cold Spring Harbor Protocols, 2020This protocol describes the standard, old-fashioned but reliable procedure for cloning linear DNA fragments whose ends are incompatible with each other but are compatible with those of the linearized vector.
Michael R, Green, Joseph, Sambrook
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Nature, 1997
What are the implications for humankind of the astounding report two weeks ago of the production of viable sheep from adult cells? The moral imperative of preserving human dignity must remain paramount.
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What are the implications for humankind of the astounding report two weeks ago of the production of viable sheep from adult cells? The moral imperative of preserving human dignity must remain paramount.
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2013
One major obstacle in realizing the potential behind human embryonic stem cells (hESC) is the availability of efficient and reliable engineering methods. Such methods require cloning technologies that can be applied to a variety of platforms and can serve multiple functions.
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One major obstacle in realizing the potential behind human embryonic stem cells (hESC) is the availability of efficient and reliable engineering methods. Such methods require cloning technologies that can be applied to a variety of platforms and can serve multiple functions.
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2004
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects.
Michael J, Lodes +3 more
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Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects.
Michael J, Lodes +3 more
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Science, 2017
Overgrowth of mutant blood cells is common in older people—and a risk factor for serious ...
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Overgrowth of mutant blood cells is common in older people—and a risk factor for serious ...
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Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
Science, 1989J. Riordan
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2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
openaire +2 more sources