Results 31 to 40 of about 538,381 (248)
Journal of Integrative Agriculture, 2013 Iron-sulfur cluster biosynthesis involving the nitrogen fixation (Nif) proteins has been proposed as a general mechanism acting in various organisms. NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses ...
Lian-jun WANG +5 more
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Cloning and gene expression of alkane hydroxylase gene from Pseudomonas putida [PDF]
مجله بیوتکنولوژی کشاورزی, 2017 Pseudomonas putida is a bacterium that has ability to growth on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other bacteria community. P. putida have alkane hydroxylase system (
Mahdi Hasanshahian, Hadi Ravan
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FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Easy method for six-fragment Golden Gate Assembly of modular vectors
BioTechniques, 2023 Efficient cloning techniques are a requirement for synthetic biology. This study provides a simplified cloning method based on Golden Gate Assembly that can be used for rapid vector construction.
Ionut Adrian Cepleanu-Pascu +3 more
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Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source
Brazilian Journal of Biology, 2021 Alpha amylase, catalyzing the hydrolysis of starch is a ubiquitous enzyme with tremendous industrial applications. A 1698 bp gene coding for 565 amino acid amylase was PCR amplified from Geobacillus thermodenitrificans DSM-465, cloned in pET21a ...
A. Al-Amri +7 more
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Cell wall target fragment discovery using a low‐cost, minimal fragment library
FEBS Letters, EarlyView.LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan +5 more
wiley +1 more source
Journal of Animal Reproduction and BiotechnologyBackground: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time.
Young-Bum Son +10 more
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Improved Method of Detection Falsification Results the Digital Image in Conditions of Attacks [PDF]
Problems of the Regional Energetics, 2016 The modern level of information technologies development has led to unheard ease embodiments hitherto unauthorized modifications of digital content. At the moment, very important question is the effective expert examination of authenticity of digital ...
Kobozeva A.A., Grigorenko S.N.
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