Results 181 to 190 of about 94,691 (233)

Pyruvate fermentation by Clostridium acetobutylicum

Biochemistry and Cell Biology, 1989
Clostridium acetobutylicum ATCC 824 using pyruvate as the sole carbon source produced mainly acetate and butyrate as end products of fermentation. Acetate and butyrate kinase activities were higher in cells growing in the presence of pyruvate than glucose, whereas the level of the acetoacetate decarboxylase, an enzyme involved in solvent formation ...
R, Janati-Idrissi, A M, Junelles, A, el Kanouni, H, Petitdemange, R, Gay   +4 more
openaire   +2 more sources

Engineering Clostridium acetobutylicum for alcohol production

Journal of Biotechnology, 2013
While Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). The adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was ...
Hou, Xiaohu, Peng, Wanfeng, Xiong, Lian, Huang, Chao, Chen, Xuefang, Chen, Xinde, Zhang, Weiguo   +6 more
openaire   +3 more sources

Butyrate as a growth factor of Clostridium acetobutylicum

bioRxiv
The butyrate biosynthetic pathway not only contributes to electron management and energy generation in butyrate forming bacteria, but also confers evolutionary advantages to the host by inhibiting the growth of surrounding butyrate-sensitive microbes ...
Hyeon-Jae Seo, Sofia H. Capece, John D. Hill, Jonathan K. Otten, E. Papoutsakis   +4 more
semanticscholar   +1 more source

Engineering Cellulase Activity into Clostridium acetobutylicum

2012
Clostridium acetobutylicum produces substantial amounts of butanol, and an engineered cellulolytic strain of the bacterium would be an attractive candidate for biofuel production using consolidated bioprocessing. Recent studies have shown that this solventogenic bacterium can be used as a host for heterologous production and secretion of individual ...
Henri-Pierre, Fierobe, Florence, Mingardon, Angélique, Chanal   +2 more
openaire   +2 more sources

Initiation of endospore formation in Clostridium acetobutylicum

Anaerobe, 2004
Endospore formation in bacilli and clostridia shows remarkable similarities in morphology as well as in physiological and molecular biological cellular events. Major differences are the formation of clostridial stage cells and granulose accumulation in clostridia. In both genera, a cascade of sigma factors is activated after septation (by help of sigma(
Peter, Dürre, Concha, Hollergschwandner
openaire   +2 more sources

Transfer of Tn1545 and Tn916 to Clostridium acetobutylicum

Plasmid, 1989
Tn1545, a conjugative transposon originally discovered in Streptococcus pneumoniae, has been transferred from Enterococcus faecalis and Bacillus subtilis to Clostridium acetobutylicum NCIB 8052. Transfer between different strains of C. acetobutylicum has also been observed. Insertion of Tn1545 into the C.
R C, Woolley, A, Pennock, R J, Ashton, A, Davies, M, Young   +4 more
openaire   +2 more sources

Fluoreszenzproteine in Clostridium acetobutylicum

2013
Derzeit gibt es nur wenige molekulare Werkzeuge, um die Genexpression biotechnologisch relevanter Clostridien gezielt zu modifizieren. Daher wurde in dieser Arbeit für den anaeroben Modellorganismus C. acetobutylicum ein neues Fluoreszenz-basiertes In-vivo-Reportersystem etabliert.
openaire   +1 more source

Fermentation of xylan by Clostridium acetobutylicum

Enzyme and Microbial Technology, 1986
Abstract The ability of Clostridium acetobutylicum to utilize hemicelluloses as a substrate for butanol production was examined. Direct fermentation of byproduct corn fibres from the wet milling process showed complete utilization of starch and 20% utilization of hemicellulose with concurrent production of solvents and organic acids. Fermentations of
S.A. Lemmel, Rathin Datta, J.R. Frankiewicz   +2 more
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Galactose transport in Clostridium acetobutylicum P262

Letters in Applied Microbiology, 1996
N.A. GUTIERREZ AND I.S. MADDOX. 1996. Evidence is provided that there are two separate systems for galactose uptake in Clostridium acetobutylicum P262, a phosphoenolpyruvate-dependent phosphotransferase system and a proton motive force-driven system. Glucose uptake involves the former but not the latter.
N.A. Gutierrez, I.S. Maddox
openaire   +1 more source

Metabolic Engineering von Clostridium acetobutylicum

2012
Eine Plasmid-vermittelte homologe Co-Expressionen der Chaperone GrpE und HtpG führten zu einer signifikant erhöhten Buntanolresistenz, jedoch nicht zu einer erhöhten Butanolproduktion in C. acetobutylicum. Zudem zeigten die untersuchten Expressionsstämme eine effizientere Adaption an sich wiederholende Butanolstress-Experimente.
openaire   +1 more source