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Separate, Separated, and Together: The Transcriptional Program of the Clostridium acetobutylicum-Clostridium Ljungdahlii Syntrophy Leading to Interspecies Cell Fusion [PDF]

open access: yesMSystems
Syntrophic cocultures (hitherto assumed to be commensalistic) of Clostridium acetobutylicum and Clostridium ljungdahlii, whereby CO2 and H2 produced by the former feed the latter, result in interspecies cell fusion involving large-scale exchange of ...
Eleftherios T Papoutsakis
exaly   +6 more sources

Pleiotropic Regulator GssR Positively Regulates Autotrophic Growth of Gas-Fermenting Clostridium ljungdahlii [PDF]

open access: yesMicroorganisms, 2023
Clostridium ljungdahlii is a representative autotrophic acetogen capable of producing multiple chemicals from one-carbon gases (CO2/CO). The metabolic and regulatory networks of this carbon-fixing bacterium are interesting, but still remain minimally ...
Huan Zhang   +6 more
doaj   +3 more sources

Cross-Talk Between Engineered Clostridium acetobutylicum and Clostridium ljungdahlii in Syntrophic Cocultures Enhances Isopropanol and Butanol Production [PDF]

open access: yesFrontiers in Microbiology
There is a need for efficient and sustainable production of essential chemicals such as isopropanol and butanol from renewable sugar feedstocks. Microbial fermentations use glycolysis, and as result, a third of the sugar carbon is lost to CO2 through ...
Eleftherios T Papoutsakis
exaly   +7 more sources

Energy Conservation and Carbon Flux Distribution During Fermentation of CO or H2/CO2 by Clostridium ljungdahlii [PDF]

open access: yesFrontiers in Microbiology, 2020
Both CO and H2 can be utilized as energy sources during the autotrophic growth of Clostridium ljungdahlii. In principle, CO is a more energetically and thermodynamically favorable energy source for gas fermentation in comparison to H2.
Fuli Li, Wen-Zhu Tang
exaly   +4 more sources

A Heterodimeric Reduced-Ferredoxin-Dependent Methylenetetrahydrofolate Reductase from Syngas-Fermenting Clostridium ljungdahlii [PDF]

open access: yesMicrobiology Spectrum, 2021
The strict anaerobe Clostridium ljungdahlii can ferment CO or H2/CO2 via the Wood-Ljungdahl pathway to acetate, ethanol, and 2,3‐butanediol. This ability has attracted considerable interest, since it can be used for syngas fermentation to produce ...
Jihong Yi   +6 more
doaj   +4 more sources

Functional dissection and modulation of the BirA protein for improved autotrophic growth of gas‐fermenting Clostridium ljungdahlii [PDF]

open access: yesMicrobial Biotechnology, 2021
Summary Gas‐fermenting Clostridium species can convert one‐carbon gases (CO2/CO) into a variety of chemicals and fuels, showing excellent application prospects in green biological manufacturing.
Can Zhang   +7 more
doaj   +2 more sources

Identifying and Engineering Bottlenecks of Autotrophic Isobutanol Formation in Recombinant C. ljungdahlii by Systemic Analysis [PDF]

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Clostridium ljungdahlii (C. ljungdahlii, CLJU) is natively endowed producing acetic acid, 2,3-butandiol, and ethanol consuming gas mixtures of CO2, CO, and H2 (syngas). Here, we present the syngas-based isobutanol formation using C. ljungdahlii harboring
Maria Hermann   +7 more
doaj   +3 more sources

A Narrow pH Range Supports Butanol, Hexanol, and Octanol Production From Syngas in a Continuous Co-Culture of Clostridium ljungdahlii and Clostridium kluyveri With In-Line Product Extraction

open access: yesFrontiers in Microbiology, 2016
Carboxydotrophic bacteria (CTB) have received attention due to their ability to synthesize commodity chemicals from producer gas and synthesis gas (syngas).
Drir M Martijn Diender   +2 more
exaly   +3 more sources

Isobutanol Production by Autotrophic Acetogenic Bacteria

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Two different isobutanol synthesis pathways were cloned into and expressed in the two model acetogenic bacteria Acetobacterium woodii and Clostridium ljungdahlii. A.
Sandra Weitz   +5 more
doaj   +1 more source

Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a Clostridium acetobutylicum/Clostridium ljungdahlii Syntrophic Coculture

open access: yesmSystems, 2021
Clostridium acetobutylicum and Clostridium ljungdahlii grown in a syntrophic culture were recently shown to fuse membranes and exchange cytosolic contents, yielding hybrid cells with significant shifts in gene expression and growth phenotypes.
Charles Foster   +3 more
doaj   +1 more source

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