Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii [PDF]
Engineering in Life SciencesSustainable chemical production from C1 gaseous substrates, such as syngas or CO2/H2, can be achieved through gas fermentation. In gas fermentation, acetogenic bacteria are able to utilize oxidized inorganic carbon sources as the sole carbon source and ...
Santiago T. Boto +3 more
doaj +5 more sources
A Heterodimeric Reduced-Ferredoxin-Dependent Methylenetetrahydrofolate Reductase from Syngas-Fermenting Clostridium ljungdahlii [PDF]
Microbiology Spectrum, 2021 The strict anaerobe Clostridium ljungdahlii can ferment CO or H2/CO2 via the Wood-Ljungdahl pathway to acetate, ethanol, and 2,3‐butanediol. This ability has attracted considerable interest, since it can be used for syngas fermentation to produce ...
Fu-Li Li, Shuning Wang
exaly +6 more sources
Energy Conservation and Carbon Flux Distribution During Fermentation of CO or H2/CO2 by Clostridium ljungdahlii [PDF]
Frontiers in Microbiology, 2020 Both CO and H2 can be utilized as energy sources during the autotrophic growth of Clostridium ljungdahlii. In principle, CO is a more energetically and thermodynamically favorable energy source for gas fermentation in comparison to H2.
Fu-Li Li
exaly +4 more sources
Pleiotropic Regulator GssR Positively Regulates Autotrophic Growth of Gas-Fermenting Clostridium ljungdahlii [PDF]
Microorganisms, 2023 Clostridium ljungdahlii is a representative autotrophic acetogen capable of producing multiple chemicals from one-carbon gases (CO2/CO). The metabolic and regulatory networks of this carbon-fixing bacterium are interesting, but still remain minimally ...
Huan Zhang +6 more
doaj +4 more sources
Separate, separated, and together: the transcriptional program of the Clostridium acetobutylicum-Clostridium ljungdahlii syntrophy leading to interspecies cell fusion [PDF]
mSystemsSyntrophic cocultures (hitherto assumed to be commensalistic) of Clostridium acetobutylicum and Clostridium ljungdahlii, whereby CO2 and H2 produced by the former feed the latter, result in interspecies cell fusion involving large-scale exchange of ...
Noah B. Willis +1 more
doaj +4 more sources
Elucidating the roles of essential genes in autotrophic metabolism and cell morphology of Clostridium ljungdahlii by CRISPRi [PDF]
Applied Microbiology and BiotechnologyUnderstanding the function of essential genes in Clostridium ljungdahlii is critical for unraveling its autotrophic metabolism and optimizing its potential as a platform for syngas fermentation.
Saira Munir +9 more
doaj +4 more sources
Functional dissection and modulation of the BirA protein for improved autotrophic growth of gas‐fermenting Clostridium ljungdahlii [PDF]
Microbial Biotechnology, 2021 Summary Gas‐fermenting Clostridium species can convert one‐carbon gases (CO2/CO) into a variety of chemicals and fuels, showing excellent application prospects in green biological manufacturing.
Can Zhang +7 more
doaj +2 more sources
Enzyme-constrained metabolic model and in silico metabolic engineering of Clostridium ljungdahlii for the development of sustainable production processes [PDF]
Computational and Structural Biotechnology Journal, 2023 Constraint-based genome-scale models (GEMs) of microorganisms provide a powerful tool for predicting and analyzing microbial phenotypes as well as for understanding how these are affected by genetic and environmental perturbations.
Antonio Caivano +3 more
doaj +2 more sources
Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii [PDF]
Biotechnology for Biofuels, 2019 Background Clostridium spp. can synthesize valuable chemicals and fuels by utilizing diverse waste-stream substrates, including starchy biomass, lignocellulose, and industrial waste gases. However, metabolic engineering in Clostridium spp. is challenging
Gabriele Philipps +2 more
doaj +2 more sources
Reducing Oxygen Stress and Improving Hydrogen Availability Boosts Microbial Electrosynthesis by Clostridium ljungdahlii. [PDF]
ChemSusChemMicrobial electrosynthesis (MES) from CO2 by acetogens enables the production of value‐added chemicals. However, its current limitations include O2 stress and insufficient H2 availability. Using Clostridium ljungdahlii as a model in 1‐L electrobioreactors providing high process control, MES is boosted to unprecedent acetate concentrations and rates ...
Kuchenbuch A +6 more
europepmc +2 more sources