Inducible promoters of bacterial microcompartments improve the CRISPR/Cas9 tools for efficient metabolic engineering of Clostridium ljungdahlii [PDF]
Applied and Environmental MicrobiologyClostridium ljungdahlii, as a model acetogen strain, represents a novel platform for biotechnological production for CO2 fixation. The genome of C. ljungdahlii harbors two gene loci associated with glycyl radical enzyme-associated microcompartments (GRMs)
Jun-Zhe Zhang +6 more
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The Rnf Complex of
mBio, 2013 It has been predicted that the Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase which contributes to ATP synthesis by an H+-translocating ATPase under both autotrophic and heterotrophic growth conditions ...
Pier-Luc Tremblay +4 more
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Biofilm Formation by Clostridium ljungdahlii Is Induced by Sodium Chloride Stress: Experimental Evaluation and Transcriptome Analysis. [PDF]
PLoS ONE, 2017 The acetogen Clostridium ljungdahlii is capable of syngas fermentation and microbial electrosynthesis. Biofilm formation could benefit both these applications, but was not yet reported for C. ljungdahlii.
Jo Philips +3 more
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Nitrate Feed Improves Growth and Ethanol Production of Clostridium ljungdahlii With CO2 and H2, but Results in Stochastic Inhibition Events [PDF]
Frontiers in Microbiology, 2020 The pH-value in fermentation broth is a critical factor for the metabolic flux and growth behavior of acetogens. A decreasing pH level throughout time due to undissociated acetic acid accumulation is anticipated under uncontrolled pH conditions such as ...
Christian-Marco Klask +4 more
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Frontiers in Microbiology, 2016 Carboxydotrophic bacteria (CTB) have received attention due to their ability to synthesize commodity chemicals from producer gas and synthesis gas (syngas).
Hanno Richter +2 more
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pH-Dependent Metabolic Regulation in Clostridium ljungdahlii During CO Fermentation
FermentationClostridium ljungdahlii is a model acetogenic bacterium utilized for ethanol production from syngas, with its growth and ethanol synthesis being profoundly influenced by fermentation pH.
Ze-Rong Liu +9 more
doaj +2 more sources
Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a Clostridium acetobutylicum/Clostridium ljungdahlii Syntrophic Coculture [PDF]
MSystems, 2021 Charles Foster +2 more
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Implementation of a Clostridium luticellarii genome-scale model for upgrading syngas fermentations [PDF]
Computational and Structural Biotechnology JournalSyngas fermentation is a powerful platform for converting waste streams into sustainable carboxylic acid precursors for value-added biochemicals. Steel mills produce significant syngas, yet industrial microbial syngas valorization remains unrealized. The
William T. Scott, Jr. +7 more
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Expanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii. [PDF]
Sci Rep, 2016 AbstractIncreasing interest in homoacetogenic bacteria for the production of biochemicals and biofuels requisites the development of new genetic tools for these atypical production organisms. An attractive host for the conversion of synthesis gas or electricity into multi-carbon compounds is Clostridium ljungdahlii.
Molitor B +4 more
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Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. [PDF]
Appl Microbiol Biotechnol, 2019 Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was ...
Annan FJ +8 more
europepmc +4 more sources