A Heterodimeric Reduced-Ferredoxin-Dependent Methylenetetrahydrofolate Reductase from Syngas-Fermenting Clostridium ljungdahlii [PDF]
Microbiology Spectrum, 2021 The strict anaerobe Clostridium ljungdahlii can ferment CO or H2/CO2 via the Wood-Ljungdahl pathway to acetate, ethanol, and 2,3‐butanediol. This ability has attracted considerable interest, since it can be used for syngas fermentation to produce ...
Jihong Yi +6 more
doaj +4 more sources
A technique for lyopreservation of Clostridium ljungdahlii in a biocomposite matrix for CO absorption. [PDF]
PLoS ONE, 2017 A system capable of biocatalytic conversion of distributed sources of single carbon gases such as carbon monoxide into hydrocarbons can be highly beneficial for developing commercially viable biotechnology applications in alternative energy.
Mark J Schulte +8 more
doaj +5 more sources
Converting Carbon Dioxide to Butyrate with an Engineered Strain of
mBio, 2014 Microbial conversion of carbon dioxide to organic commodities via syngas metabolism or microbial electrosynthesis is an attractive option for production of renewable biocommodities.
Toshiyuki Ueki +3 more
doaj +5 more sources
Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO2 and H2 [PDF]
Microbial Cell Factories, 2022 Background The replacement of fossil fuels and petrochemicals with sustainable alternatives is necessary to mitigate the effects of climate change and also to counteract diminishing fossil resources. Acetogenic microorganisms such as Clostridium spp. are
Ira Lauer +2 more
doaj +4 more sources
Elucidating the roles of essential genes in autotrophic metabolism and cell morphology of Clostridium ljungdahlii by CRISPRi [PDF]
Applied Microbiology and BiotechnologyUnderstanding the function of essential genes in Clostridium ljungdahlii is critical for unraveling its autotrophic metabolism and optimizing its potential as a platform for syngas fermentation.
Saira Munir +9 more
doaj +4 more sources
Cross-talk between engineered Clostridium acetobutylicum and Clostridium ljungdahlii in syntrophic cocultures enhances isopropanol and butanol production [PDF]
Frontiers in MicrobiologyThere is a need for efficient and sustainable production of essential chemicals such as isopropanol and butanol from renewable sugar feedstocks. Microbial fermentations use glycolysis, and as result, a third of the sugar carbon is lost to CO2 through ...
Jonathan K. Otten +10 more
doaj +5 more sources
Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a
mSystems, 2021 Clostridium acetobutylicum and Clostridium ljungdahlii grown in a syntrophic culture were recently shown to fuse membranes and exchange cytosolic contents, yielding hybrid cells with significant shifts in gene expression and growth phenotypes.
Charles Foster +3 more
doaj +2 more sources
Enzyme-constrained metabolic model and in silico metabolic engineering of Clostridium ljungdahlii for the development of sustainable production processes [PDF]
Computational and Structural Biotechnology Journal, 2023 Constraint-based genome-scale models (GEMs) of microorganisms provide a powerful tool for predicting and analyzing microbial phenotypes as well as for understanding how these are affected by genetic and environmental perturbations.
Antonio Caivano +3 more
doaj +2 more sources
Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins [PDF]
Applied and Environmental Microbiology, 2020 Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes. Green fluorescent protein (GFP), mCherry, and flavin-binding proteins (and their derivatives) have been used only in a few clostridia with limited success and ...
Eleftherios Papoutsakis
exaly +5 more sources
Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii [PDF]
Biotechnology for Biofuels, 2019 Background Clostridium spp. can synthesize valuable chemicals and fuels by utilizing diverse waste-stream substrates, including starchy biomass, lignocellulose, and industrial waste gases. However, metabolic engineering in Clostridium spp. is challenging
Gabriele Philipps +2 more
doaj +2 more sources