Enzyme-constrained metabolic model and in silico metabolic engineering of Clostridium ljungdahlii for the development of sustainable production processes [PDF]
Computational and Structural Biotechnology Journal, 2023 Constraint-based genome-scale models (GEMs) of microorganisms provide a powerful tool for predicting and analyzing microbial phenotypes as well as for understanding how these are affected by genetic and environmental perturbations.
Antonio Caivano +3 more
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Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii [PDF]
Biotechnology for Biofuels, 2019 Background Clostridium spp. can synthesize valuable chemicals and fuels by utilizing diverse waste-stream substrates, including starchy biomass, lignocellulose, and industrial waste gases. However, metabolic engineering in Clostridium spp. is challenging
Gabriele Philipps +2 more
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Elucidating the roles of essential genes in autotrophic metabolism and cell morphology of Clostridium ljungdahlii by CRISPRi [PDF]
Applied Microbiology and BiotechnologyUnderstanding the function of essential genes in Clostridium ljungdahlii is critical for unraveling its autotrophic metabolism and optimizing its potential as a platform for syngas fermentation.
Saira Munir +9 more
doaj +3 more sources
Biofilm Formation by Clostridium ljungdahlii Is Induced by Sodium Chloride Stress: Experimental Evaluation and Transcriptome Analysis. [PDF]
PLoS ONE, 2017 The acetogen Clostridium ljungdahlii is capable of syngas fermentation and microbial electrosynthesis. Biofilm formation could benefit both these applications, but was not yet reported for C. ljungdahlii.
Jo Philips +3 more
doaj +4 more sources
A Narrow pH Range Supports Butanol, Hexanol, and Octanol Production from Syngas in a Continuous Co-culture of Clostridium ljungdahlii and Clostridium kluyveri with In-Line Product Extraction [PDF]
Frontiers in Microbiology, 2016 Carboxydotrophic bacteria (CTB) have received attention due to their ability to synthesize commodity chemicals from producer gas and synthesis gas (syngas).
Martijn Diender +2 more
exaly +4 more sources
A Heterodimeric Reduced-Ferredoxin-Dependent Methylenetetrahydrofolate Reductase from Syngas-Fermenting Clostridium ljungdahlii [PDF]
Microbiology Spectrum, 2021 The strict anaerobe Clostridium ljungdahlii can ferment CO or H2/CO2 via the Wood-Ljungdahl pathway to acetate, ethanol, and 2,3‐butanediol. This ability has attracted considerable interest, since it can be used for syngas fermentation to produce ...
Jihong Yi +6 more
doaj +2 more sources
Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a
mSystems, 2021 Clostridium acetobutylicum and Clostridium ljungdahlii grown in a syntrophic culture were recently shown to fuse membranes and exchange cytosolic contents, yielding hybrid cells with significant shifts in gene expression and growth phenotypes.
Charles Foster +3 more
doaj +2 more sources
Reducing Oxygen Stress and Improving Hydrogen Availability Boosts Microbial Electrosynthesis by Clostridium ljungdahlii. [PDF]
ChemSusChemMicrobial electrosynthesis (MES) from CO2 by acetogens enables the production of value‐added chemicals. However, its current limitations include O2 stress and insufficient H2 availability. Using Clostridium ljungdahlii as a model in 1‐L electrobioreactors providing high process control, MES is boosted to unprecedent acetate concentrations and rates ...
Kuchenbuch A +6 more
europepmc +2 more sources
Inducible promoters of bacterial microcompartments improve the CRISPR/Cas9 tools for efficient metabolic engineering of Clostridium ljungdahlii [PDF]
Applied and Environmental MicrobiologyClostridium ljungdahlii, as a model acetogen strain, represents a novel platform for biotechnological production for CO2 fixation. The genome of C. ljungdahlii harbors two gene loci associated with glycyl radical enzyme-associated microcompartments (GRMs)
Jun-Zhe Zhang +6 more
doaj +2 more sources
Converting Carbon Dioxide to Butyrate with an Engineered Strain of
mBio, 2014 Microbial conversion of carbon dioxide to organic commodities via syngas metabolism or microbial electrosynthesis is an attractive option for production of renewable biocommodities.
Toshiyuki Ueki +3 more
doaj +2 more sources