The potential of caproate (hexanoate) production using Clostridium kluyveri syntrophic cocultures with Clostridium acetobutylicum or Clostridium saccharolyticum [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Caproate (hexanoate) and other medium-chain fatty acids are valuable platform chemicals produced by processes utilizing petroleum or plant oil. Clostridium kluyveri, growing on short chain alcohols (notably ethanol) and carboxylic acids (such as acetate)
Eleftherios Papoutsakis +1 more
exaly +4 more sources
Clostridium acetobutylicum Biofilm: Advances in Understanding the Basis [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Clostridium acetobutylicum is an important industrial platform capable of producing a variety of biofuels and bulk chemicals. Biofilm of C. acetobutylicum renders many production advantages and has been long and extensively applied in fermentation ...
Huifang Zhang +9 more
doaj +5 more sources
Separate, separated, and together: the transcriptional program of the Clostridium acetobutylicum-Clostridium ljungdahlii syntrophy leading to interspecies cell fusion [PDF]
MSystemsSyntrophic cocultures (hitherto assumed to be commensalistic) of Clostridium acetobutylicum and Clostridium ljungdahlii, whereby CO2 and H2 produced by the former feed the latter, result in interspecies cell fusion involving large-scale exchange of ...
Eleftherios Papoutsakis
exaly +4 more sources
Design and validation of a multiplex PCR method for the simultaneous quantification of Clostridium acetobutylicum, Clostridium carboxidivorans and Clostridium cellulovorans [PDF]
Scientific Reports, 2023 Co-cultures of clostridia with distinct physiological properties have emerged as an alternative to increase the production of butanol and other added-value compounds from biomass.
Laura Feliu-Paradeda +2 more
doaj +2 more sources
Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum [PDF]
Nature Communications, 2022 Ferredoxin-NAD(P) + oxidoreductases are important enzymes for redox balancing in n-butanol production by Clostridium acetobutylicum, but the encoding genes remain unknown.
Céline Foulquier +10 more
doaj +2 more sources
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and
Yu-Sin Jang +7 more
doaj +2 more sources
Identification and regulation of an alternative PTS for disaccharide utilization in Clostridium acetobutylicum [PDF]
Applied and Environmental MicrobiologyClostridium acetobutylicum is an important solventogenic bacterium capable of acetone-butanol-ethanol fermentation by utilizing a variety of carbon sources.
Zhenxing Ren +4 more
doaj +2 more sources
Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a
mSystems, 2021 Clostridium acetobutylicum and Clostridium ljungdahlii grown in a syntrophic culture were recently shown to fuse membranes and exchange cytosolic contents, yielding hybrid cells with significant shifts in gene expression and growth phenotypes.
Charles Foster +3 more
doaj +2 more sources
From pre-culture to solvent: current trends in Clostridium acetobutylicum cultivation [PDF]
Applied Microbiology and BiotechnologyThe biological production of butanol via ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum has a storied history of over 100 years, initially driven by the demand for synthetic rubber during World War I and later for industrial ...
Katharina Oehlenschläger +3 more
doaj +2 more sources
Cross-talk between engineered Clostridium acetobutylicum and Clostridium ljungdahlii in syntrophic cocultures enhances isopropanol and butanol production [PDF]
Frontiers in MicrobiologyThere is a need for efficient and sustainable production of essential chemicals such as isopropanol and butanol from renewable sugar feedstocks. Microbial fermentations use glycolysis, and as result, a third of the sugar carbon is lost to CO2 through ...
Jonathan K. Otten +10 more
doaj +2 more sources