The potential of caproate (hexanoate) production using Clostridium kluyveri syntrophic cocultures with Clostridium acetobutylicum or Clostridium saccharolyticum [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Caproate (hexanoate) and other medium-chain fatty acids are valuable platform chemicals produced by processes utilizing petroleum or plant oil. Clostridium kluyveri, growing on short chain alcohols (notably ethanol) and carboxylic acids (such as acetate)
Eleftherios Papoutsakis +1 more
exaly +5 more sources
Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum [PDF]
Nature Communications, 2022 Ferredoxin-NAD(P) + oxidoreductases are important enzymes for redox balancing in n-butanol production by Clostridium acetobutylicum, but the encoding genes remain unknown.
Céline Foulquier +10 more
doaj +3 more sources
From pre-culture to solvent: current trends in Clostridium acetobutylicum cultivation [PDF]
Applied Microbiology and BiotechnologyThe biological production of butanol via ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum has a storied history of over 100 years, initially driven by the demand for synthetic rubber during World War I and later for industrial ...
Katharina Oehlenschläger +3 more
doaj +3 more sources
Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a
mSystems, 2021 Clostridium acetobutylicum and Clostridium ljungdahlii grown in a syntrophic culture were recently shown to fuse membranes and exchange cytosolic contents, yielding hybrid cells with significant shifts in gene expression and growth phenotypes.
Charles Foster +3 more
doaj +3 more sources
Separate, separated, and together: the transcriptional program of the Clostridium acetobutylicum-Clostridium ljungdahlii syntrophy leading to interspecies cell fusion [PDF]
MSystemsSyntrophic cocultures (hitherto assumed to be commensalistic) of Clostridium acetobutylicum and Clostridium ljungdahlii, whereby CO2 and H2 produced by the former feed the latter, result in interspecies cell fusion involving large-scale exchange of ...
Eleftherios Papoutsakis
exaly +4 more sources
The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation [PDF]
Nature Communications, 2017 Polyketides are secondary metabolites mainly found in aerobic organisms with wide applications in medicine and agriculture. Here, the authors uncover new polyketides native to the anaerobic bacterium Clostridium acetobutylicum and show their role in ...
Nicolaus A. Herman +5 more
doaj +3 more sources
Co‑cultivation of anaerobic fungi with Clostridium acetobutylicum bolsters butyrate and butanol production from cellulose and lignocellulose. [PDF]
J Ind Microbiol Biotechnol, 2023 A system for co-cultivation of anaerobic fungi with anaerobic bacteria was established based on lactate cross-feeding to produce butyrate and butanol from plant biomass.
Brown JL +10 more
europepmc +2 more sources
Clostridium acetobutylicum Biofilm: Advances in Understanding the Basis [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Clostridium acetobutylicum is an important industrial platform capable of producing a variety of biofuels and bulk chemicals. Biofilm of C. acetobutylicum renders many production advantages and has been long and extensively applied in fermentation ...
Huifang Zhang +9 more
doaj +2 more sources
Improved CRISPR/Cas9 Tools for the Rapid Metabolic Engineering of Clostridium acetobutylicum. [PDF]
Int J Mol Sci, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas (CRISPR-associated proteins)9 tools have revolutionized biology—several highly efficient tools have been constructed that have resulted in the ability to quickly engineer model ...
Wilding-Steele T +3 more
europepmc +2 more sources
Design and validation of a multiplex PCR method for the simultaneous quantification of Clostridium acetobutylicum, Clostridium carboxidivorans and Clostridium cellulovorans [PDF]
Scientific Reports, 2023 Co-cultures of clostridia with distinct physiological properties have emerged as an alternative to increase the production of butanol and other added-value compounds from biomass.
Laura Feliu-Paradeda +2 more
doaj +2 more sources