An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors [PDF]
Biotechnology for Biofuels, 2019 Background Clostridium acetobutylicum and Clostridium saccharobutylicum are Gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and ethanol).
Celine Foulquier +10 more
doaj +2 more sources
Frontiers in Microbiology, 2015 Autolysis is a widespread phenomenon in bacteria. In batch fermentation of Clostridium acetobutylicum ATCC 824, there is a spontaneous large-scale autolysis phenomenon with significant decrease of cell density immediately after exponential phase.
Fu-Li Li, Li Fu-Li
exaly +3 more sources
Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins [PDF]
Biotechnology for Biofuels, 2018 Background Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS). The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce
Dong Liu +6 more
doaj +2 more sources
Synergies revealed: RNA-seq study of C. acetobutylicum and C. carboxidivorans co-cultured in the presence of conductive materials [PDF]
Microbiology SpectrumCo-cultures can improve substrate utilization and product yields, yet the dynamics between species remain highly variable and poorly understood. In this study, we investigated the metabolic and transcriptional interplay between Clostridium acetobutylicum
L. Feliu-Paradeda +6 more
doaj +2 more sources
Unveiling a catalytically promiscuous feruloyl esterase from Clostridium acetobutylicum [PDF]
Applied Microbiology and BiotechnologyClostridium species have garnered increasing attention for their ability to convert lignocellulosic biomass into renewable fuels and platform chemicals.
Shang Li +7 more
doaj +2 more sources
The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation [PDF]
Nature Communications, 2017 Polyketides are secondary metabolites mainly found in aerobic organisms with wide applications in medicine and agriculture. Here, the authors uncover new polyketides native to the anaerobic bacterium Clostridium acetobutylicum and show their role in ...
Nicolaus A. Herman +5 more
doaj +2 more sources
Diauxic growth of Clostridium acetobutylicum ATCC 824 when grown on mixtures of glucose and cellobiose [PDF]
AMB Express, 2018 Clostridium acetobutylicum, a promising organism for biomass transformation, has the capacity to utilize a wide variety of carbon sources. During pre-treatments of (ligno) cellulose through thermic and/or enzymatic processes, complex mixtures of oligo ...
Felipe Buendia-Kandia +5 more
doaj +2 more sources
The significance of aspartate on NAD(H) biosynthesis and ABE fermentation in Clostridium acetobutylicum ATCC 824 [PDF]
AMB Express, 2019 The co-factor NADH plays an important role in butanol biosynthesis. In this study, we found that aspartate could effectively improve the butanol production of Clostridium acetobutylicum ATCC 824.
Zhengping Liao +3 more
doaj +2 more sources
Metabolic interplay in a synthetic consortium: insights into the mediating role of a minority species [PDF]
Frontiers in MicrobiologyMicrobial interactions are pivotal components of Earth's ecosystems, driving essential processes that sustain life, regulate environmental conditions, and ensure ecosystem resilience. A comprehensive understanding of these relationships is imperative for
Cassandra Backes +5 more
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Effects of butanol on Clostridium acetobutylicum [PDF]
Applied and Environmental Microbiology, 1985 The internal pH of Clostridium acetobutylicum was determined at various stages during the growth of the organism. Even in the presence of significant quantities of acetic, butyric, and lactic acids, an internal pH of 6.2 was maintained. Experiments using N,N'-dicyclohexylcarbodiimide indicated that a functioning H+-ATPase is necessary for internal pH ...
L K, Bowles, W L, Ellefson
openaire +2 more sources