Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins. [PDF]
Appl Environ Microbiol, 2020 Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes.
Charubin K, Streett H, Papoutsakis ET.
europepmc +2 more sources
An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors [PDF]
Biotechnology for Biofuels, 2019 Background Clostridium acetobutylicum and Clostridium saccharobutylicum are Gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and ethanol).
Celine Foulquier +10 more
doaj +2 more sources
Continuous Butanol Fermentation of Dilute Acid-Pretreated De-oiled Rice Bran by Clostridium acetobutylicum YM1. [PDF]
Sci Rep, 2019 Continuous fermentation of dilute acid-pretreated de-oiled rice bran (DRB) to butanol by the Clostridium acetobutylicum YM1 strain was investigated. Pretreatment of DRB with dilute sulfuric acid (1%) resulted in the production of 42.12 g/L total sugars ...
Al-Shorgani NKN +5 more
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Frontiers in Microbiology, 2015 Autolysis is a widespread phenomenon in bacteria. In batch fermentation of Clostridium acetobutylicum ATCC 824, there is a spontaneous large-scale autolysis phenomenon with significant decrease of cell density immediately after exponential phase.
Fu-Li Li
exaly +3 more sources
Butyrate as a growth factor of Clostridium acetobutylicum
Metabolic EngineeringAbstract The butyrate biosynthetic pathway not only contributes to electron management and energy generation in butyrate forming bacteria, but also confers evolutionary advantages to the host by inhibiting the growth of surrounding butyrate-sensitive microbes.
Hyeongmin Seo +4 more
openaire +3 more sources
Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins [PDF]
Biotechnology for Biofuels, 2018 Background Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS). The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce
Dong Liu +6 more
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Unveiling a catalytically promiscuous feruloyl esterase from Clostridium acetobutylicum [PDF]
Applied Microbiology and BiotechnologyClostridium species have garnered increasing attention for their ability to convert lignocellulosic biomass into renewable fuels and platform chemicals.
Shang Li +7 more
doaj +2 more sources
Pleiotropic regulation of a glucose-specific PTS in Clostridium acetobutylicum for high-efficient butanol production from corn stover without detoxification. [PDF]
Biotechnol Biofuels, 2019 BackgroundCorn stover (CS) is evaluated as the most favorable candidate feedstock for butanol production via microbial acetone–butanol–ethanol (ABE) fermentation by Clostridium acetobutylicum.
Wu Y +6 more
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Co-production of solvents and organic acids in butanol fermentation by Clostridium acetobutylicum in the presence of lignin-derived phenolics. [PDF]
RSC Adv, 2019 Co-production of solvents (butanol, acetone, and ethanol) and organic acids (butyrate and acetate) by Clostridium acetobutylicum using lignocellulosic biomass as a substrate could further enlarge the application scope of butanol fermentation.
Luo H +7 more
europepmc +2 more sources
BMC Genomics, 2012 Background Clostridium acetobutylicum has been used to produce butanol in industry. Catabolite control protein A (CcpA), known to mediate carbon catabolite repression (CCR) in low GC gram-positive bacteria, has been identified and characterized in C ...
Ren Cong +6 more
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