Results 31 to 40 of about 766 (130)

Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. [PDF]

Appl Microbiol Biotechnol, 2019
Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was ...
Annan FJ, Al-Sinawi B, Humphreys CM, Norman R, Winzer K, Köpke M, Simpson SD, Minton NP, Henstra AM.   +8 more
europepmc   +4 more sources

Bioadaptive Ni single atoms unlock high rate microbial electrosynthesis of isopropanol from CO2 [PDF]

Nature Communications
Hybrid systems that integrate electrochemical CO2 reduction with microbial upgrading offer a viable route to high value organic compounds from CO2 at ambient conditions.
Guangye Zhou, Jonathan R. Humphreys, Dongfang Cheng, Shan Jiang, Wei-Ling Huang, Guangming Cheng, Nan Yao, Wei Xiong, Jeffrey T. Miller, Katherine Chou, Johannes B. M. Klok, Hongxu Chen, James Christopher Dykstra, Zhiyong Jason Ren   +13 more
doaj   +2 more sources

Metabolic response of Clostridium ljungdahlii to oxygen exposure. [PDF]

Appl Environ Microbiol, 2015
ABSTRACTClostridium ljungdahliiis an important synthesis gas-fermenting bacterium used in the biofuels industry, and a preliminary investigation showed that it has some tolerance to oxygen when cultured in rich mixotrophic medium. Batch cultures not only continue to grow and consume H2, CO, and fructose after 8% O2exposure, but fermentation product ...
Whitham JM, Tirado-Acevedo O, Chinn MS, Pawlak JJ, Grunden AM.   +4 more
europepmc   +4 more sources

Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii. [PDF]

Appl Environ Microbiol, 2014
ABSTRACT The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic ...
Banerjee A, Leang C, Ueki T, Nevin KP, Lovley DR.   +4 more
europepmc   +4 more sources

Isobutanol Production by Autotrophic Acetogenic Bacteria

Frontiers in Bioengineering and Biotechnology, 2021
Two different isobutanol synthesis pathways were cloned into and expressed in the two model acetogenic bacteria Acetobacterium woodii and Clostridium ljungdahlii. A.
Sandra Weitz, Maria Hermann, Sonja Linder, Frank R. Bengelsdorf, Ralf Takors, Peter Dürre   +5 more
doaj   +1 more source

Specific detection of “Clostridium autoethanogenum”, Clostridium ljungdahlii and Clostridium carboxidivorans in complex bioreactor samples [PDF]

FEMS Microbiology Letters, 2018
The high genetic similarity between some carboxydotrophic bacteria does not allow for the use of common sequencing techniques targeting the 16S rRNA gene for species identification. 16S rRNA sequencing fails to discriminate among Clostridium ljungdahlii and 'Clostridium autoethanogenum', despite this two species exhibit significant differences in CO2 ...
Ester Cantos-Parra, Sara Ramió-Pujol, Jesús Colprim, Sebastià Puig, Lluís Bañeras   +4 more
openaire   +2 more sources

Identifying and Engineering Bottlenecks of Autotrophic Isobutanol Formation in Recombinant C. ljungdahlii by Systemic Analysis

Frontiers in Bioengineering and Biotechnology, 2021
Clostridium ljungdahlii (C. ljungdahlii, CLJU) is natively endowed producing acetic acid, 2,3-butandiol, and ethanol consuming gas mixtures of CO2, CO, and H2 (syngas). Here, we present the syngas-based isobutanol formation using C. ljungdahlii harboring
Maria Hermann, Attila Teleki, Sandra Weitz, Alexander Niess, Andreas Freund, Frank Robert Bengelsdorf, Peter Dürre, Ralf Takors   +7 more
doaj   +1 more source

Occurrence of ferredoxin:NAD+ oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria [PDF]

PeerJ, 2016
A ferredoxin:NAD+ oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium Acetobacterium woodii. The enzyme is assumed to be encoded by the rnf genes.
Verena Hess, Rene Gallegos, J Andrew Jones, Blanca Barquera, Michael H Malamy, Volker Müller   +5 more
doaj   +2 more sources

Interactive Regulation of Formate Dehydrogenase during CO₂ Fixation in Gas-Fermenting Bacteria

mBio, 2020
Protein lysine acetylation, a prevalent posttranslational modification, regulates numerous crucial biological processes in cells. Nevertheless, how lysine acetylation interacts with other types of regulation to coordinate metabolism remains largely ...
Lu Zhang, Yanqiang Liu, Ran Zhao, Can Zhang, Weihong Jiang, Yang Gu   +5 more
doaj   +1 more source

Screening of Gas Substrate and Medium Effects on 2,3-Butanediol Production with C. ljungdahlii and C. autoethanogenum Aided by Improved Autotrophic Cultivation Technique

Fermentation, 2021
Gas fermentation by acetogens of the genus Clostridium is an attractive technology since it affords the production of biochemicals and biofuels from industrial waste gases while contributing to mitigate the carbon cycle alterations.
Luca Ricci, Valeria Agostino, Debora Fino, Angela Re   +3 more
doaj   +1 more source