Endogenous CRISPR/Cas Systems for Genome Engineering in the Acetogens Acetobacterium woodii and Clostridium autoethanogenum [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Acetogenic bacteria can play a major role in achieving Net Zero through their ability to convert CO2 into industrially relevant chemicals and fuels. Full exploitation of this potential will be reliant on effective metabolic engineering tools, such as ...
Klaus Winzer +2 more
exaly +4 more sources
Dietary Supplementation With Clostridium autoethanogenum Protein Improves Growth Performance and Promotes Muscle Protein Synthesis by Activating the mTOR Signaling Pathway of the Broiler [PDF]
Frontiers in Veterinary ScienceThe experiment aimed to evaluate the effects of different ratios of Clostridium autoethanogenum protein (CAP) used in the diets on the growth performance, muscle quality, serum indexes, and mTOR pathway of white feather broilers.
Chunqiao Shan +7 more
exaly +4 more sources
The role of ethanol oxidation during carboxydotrophic growth of Clostridium autoethanogenum [PDF]
Microbial Biotechnology, 2023 The Wood–Ljungdahl pathway is an ancient metabolic route used by acetogenic carboxydotrophs to convert CO into acetate, and some cases ethanol. When produced, ethanol is generally seen as an end product of acetogenic metabolism, but here we show that it ...
Martijn Diender +5 more
doaj +2 more sources
Clostridium autoethanogenum isopropanol production via native plasmid pCA replicon [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Clostridium autoethanogenum is a model gas-fermenting acetogen for commercial ethanol production. It is also a platform organism being developed for the carbon-negative production of acetone and isopropanol by gas fermentation. We have assembled a 5.5 kb
Robert Nogle +15 more
doaj +2 more sources
Metabolic Engineering Interventions for Sustainable 2,3-Butanediol Production in Gas-Fermenting Clostridium autoethanogenum [PDF]
mSystems, 2022 Gas fermentation provides a promising platform to turn low-cost and readily available single-carbon waste gases into commodity chemicals, such as 2,3-butanediol.
Parsa Ghadermazi +3 more
doaj +2 more sources
Absolute Proteome Quantification in the Gas-Fermenting Acetogen Clostridium autoethanogenum [PDF]
mSystems, 2022 Microbes that can recycle one-carbon (C1) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO2 and CO.
Kaspar Valgepea +11 more
doaj +2 more sources
Replacement of Dietary Fishmeal with Clostridium autoethanogenum Protein on Lipidomics and Lipid Metabolism in Muscle of Pearl Gentian Grouper [PDF]
Aquaculture Nutrition, 2023 Clostridium autoethanogenum protein (CAP) is an economical and alternative protein source. Here, three experimental diets were formulated with CAP replacing 0% (CAP-0), 30% (CAP-30), and 60% (CAP-60) of fishmeal to investigate the alterations of ...
Jia Xu +6 more
doaj +2 more sources
Autotrophic and Mixotrophic Batch Processes with Clostridium autoethanogenum LAbrini in Stirred Tank Bioreactors with Continuous Gassing [PDF]
MicroorganismsSimultaneous conversion of syngas and sugars is a promising approach to overcome limitations of syngas fermentation. Clostridium autoethanogenum LAbrini, obtained by adaptive laboratory evolution, is known to show improved autotrophic process performance.
Anne Oppelt +3 more
doaj +2 more sources
Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO [PDF]
Microbial Cell Factories, 2022 Background Ethyl acetate is a bulk chemical traditionally produced via energy intensive chemical esterification. Microbial production of this compound offers promise as a more sustainable alternative process.
James C. Dykstra +7 more
doaj +2 more sources
Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes. They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix.
François M. Seys +8 more
doaj +2 more sources