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Cloning and expression of a gene encoding the flagellin of Clostridium chauvoei
Veterinary Microbiology, 2000Clostridium chauvoei is a causative agent of blackleg and the major protective antigen of the organism is the flagellar protein. Using an Escherichia coli expression library of the C. chauvoei Okinawa strain, we isolated the fliC gene encoding the flagellin protein.
A, Kojima +6 more
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Immunological Differences Between Strains of Clostridium chauvoei
Research in Veterinary Science, 1967SUMMARY Using sheep, guinea-pigs and mice as test animals, the protective effect 0/9 straws of Cl. chauvoei was studied. The animals showed a greater degree of resistance to challenge by the homologous strain than by heterologous strains. This effect was most evident at the lower levels of immunity and the greater the level of immunity the more cross-
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Clostridium chauvoei ‐associated meningoencephalitis in a calf
Veterinary Record, 2016Meningoencephalitis in a calf associated with Clostridium chauvoei infection Bovine papular stomatitis in ...
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An indirect hemagglutination test for the detection of antibodies to clostridium chauvoei
Veterinary Microbiology, 1985An indirect hemagglutination (IHA) test using sonicated extract as the antigen was developed for the detection of antibodies to Clostridium chauvoei. This antigen can be adsorbed onto glutaraldehyde-fixed sheep red blood cells treated with tannic acid and can be destroyed by trypsin and heat treatment.
Y, Tamura, H, Makie, S, Tanaka
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Characteristics and Immunizing Properties of Culture Filtrates of Clostridium chauvoei
American Journal of Veterinary Research, 1972SUMMARY Clostridium chauvoei culture filtrate and the effects of its antigens in vaccinated hamsters and guinea pigs were studied. The immunogenic potential of hemolysin and edema-producing factor was investigated, particularly as related to the development of a valid potency test of commercial bacterins containing C. chauvoei.
K D, Claus, M E, Macheak
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Studies with Clostridium Chauvoei (Blackleg) Aggressin,
The Journal of Immunology, 1940Summary Natural aggressin, culture filtrate and sonic extract of Cl. chauvoei reduced phagocytosis in vitro when present in the phagocytic mixtures. By allowing the aggressin to act upon serum and upon leucocytes it was shown that the inhibition of phagocytosis was due to fixation of opsonins and not to a direct action upon the ...
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Clostridium chauvoei: Immunological characterization of Antigenic Preparations
Anaerobe, 1999Abstract Clostridium chauvoei is an anaerobic and sporulated bacterium which produces blackleg in cattle, sheep and other ungulates. Elsewhere we have studied the immunogenic power of the following antigenic preparations in mice: cellular extract (CE), a suspension of flagella (F), formolinized cells (FC) and heated cells (ΘC), and the immunogenic ...
Maria Silvia Di Genaro +2 more
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Extracellular proteins of Clostridium chauvoei are protective in a mouse model
Acta Veterinaria Hungarica, 2007The anaerobic bacillus Clostridium chauvoei is the causative agent of blackleg, a lethal disease that has an important impact on the sheep and cattle industry worldwide. Immunity to C. chauvoei is considered to be mainly anticellular, and for this reason there is scarce information about the immunogenicity of extracellular proteins.
Mattar, María +2 more
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Purification and sensitivity of Clostridium chauvoei hemolysin to various erythrocytes
Comparative Immunology, Microbiology and Infectious Diseases, 2006Using ammonium sulphate fractionation, the Clostridium chauvoei hemolysin was purified by cation exchange chromatography and sephacryl S-100 gel filtration. The molecular mass of the hemolysin, determined by SDS-PAGE was found to be approximately 27kDa.
Bernard, Mudenda Hang'ombe +3 more
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Blackleg (Clostridium chauvoei infection) in beef calves
The Bovine Practitioner, 2009Blackleg (Clostridium chauvoei infection) has long been recognized as a cause of death in calves grazing summer pastures. Clinical signs are not often observed in affected calves due to the peracute nature of the disease, but may include fever, lameness, and swelling and crepitation over large muscle groups, followed by collapse and death.
Daly, Russell F. +3 more
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