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Influence of process parameters on growth of Clostridium ljungdahlii and Clostridium autoethanogenum on synthesis gas

Enzyme and Microbial Technology, 2009
Effects of initial medium pH and gas flow rate on Clostridium ljungdahlii and Clostridium autoethanogenum in liquid batch, continuous gas fermentations were investigated. Synthesis gas components were supplied at varying flow rates (5, 7.5 and 10 mL/min) for C. ljungdahlii (pH 6.8 and 5.5) and C. autoethanogenum (pH 6.0).
Jacqueline L. Cotter   +2 more
openaire   +1 more source

Enhancement of bioethanol production in syngas fermentation with Clostridium ljungdahlii using nanoparticles

Bioresource Technology, 2014
In this study, nanoparticles were used to enhance bioethanol production in syngas fermentation by Clostridium ljungdahlii. Six types of nanoparticles were tested: palladium on carbon, palladium on alumina, silica, hydroxyl-functionalized single-walled carbon nanotubes, alumina, and iron(III) oxide. Of these, silica nanoparticles at a concentration of 0.
Young-Kee, Kim   +3 more
openaire   +2 more sources

Rediverting carbon flux in Clostridium ljungdahlii using CRISPR interference (CRISPRi)

Metabolic Engineering, 2018
Clostridium ljungdahlii has emerged as an attractive candidate for the bioconversion of synthesis gas (CO, CO2, H2) to a variety of fuels and chemicals through the Wood-Ljungdahl pathway. However, metabolic engineering and pathway elucidation in this microbe is limited by the lack of genetic tools to downregulate target genes. To overcome this obstacle,
Benjamin M, Woolston   +3 more
openaire   +2 more sources

Engineering Clostridium ljungdahlii as the gas-fermenting cell factory for the production of biofuels and biochemicals

Current Opinion in Chemical Biology, 2020
Clostridium ljungdahlii is a representative autotrophic gas-fermenting acetogen capable of converting CO2 and CO into biomass and multiple metabolites. The carbon fixation and conversion based on C. ljungdahlii have great potential for the sustainable production of bulk biochemicals and biofuels using industrial syngas and waste gases. With substantial
Lu, Zhang   +4 more
openaire   +2 more sources

Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain

Applied Microbiology and Biotechnology, 2016
A Clostridium ljungdahlii lab-isolated spontaneous-mutant strain, OTA1, has been shown to produce twice as much ethanol as the C. ljungdahlii ATCC 55383 strain when cultured in a mixotrophic medium containing fructose and syngas. Whole-genome sequencing identified four unique single nucleotide polymorphisms (SNPs) in the C.
Jason M Whitham   +2 more
exaly   +4 more sources

Towards product diversification in carbon negative bioprocesses with Clostridium ljungdahlii

2023
The way to sustainable chemical production will require carbon capture technologies that allow abundant and cheap carbon dioxide to be available, to be used in carbon fixation processes. Among the non-photosynthetic biological carbon fixation processes, the ones catalyzed by acetogenic bacteria such as gas fermentation and microbial electrosynthesis ...
openaire   +1 more source

Clostridium ljungdahliifor production of biofuel from synthesis gas

Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 2016
ABSTRACTLowering the redox potential of the growth medium is a critical step in the cultivation of obligate anaerobes for production of biofuels from synthesis gas. In this study, the simultaneous effects of reducing solutions (sodium sulfide and/or cysteine-HCl) and initial medium pH on the fermentation of synthesis gas using Clostridium ljungdahlii ...
M. Mohammadi   +4 more
openaire   +1 more source

Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528

Enzyme and Microbial Technology, 2015
Acetoin reductase catalyzes the formation of 2,3-butanediol from acetoin. In Clostridium ljungdahlii DSM 13528, the gene CLJU_c23220 encoding the putative Zn(2+)-dependent alcohol dehydrogenase was cloned and expressed in Escherichia coli. The recombinant enzyme, CLAR, can catalyze the conversion of acetoin to 2,3-butanediol with NADPH as the cofactor.
Yang, Tan   +3 more
openaire   +2 more sources

Clostridium ljungdahlii: A Review of the Development of an Industrial Biocatalyst

Current Biotechnology, 2016
Jason M. Whitham   +2 more
openaire   +1 more source

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