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In silico metabolic engineering of Clostridium ljungdahlii for synthesis gas fermentation
Metabolic Engineering, 2016Synthesis gas fermentation is one of the most promising routes to convert synthesis gas (syngas; mainly comprised of H2 and CO) to renewable liquid fuels and chemicals by specialized bacteria. The most commonly studied syngas fermenting bacterium is Clostridium ljungdahlii, which produces acetate and ethanol as its primary metabolic byproducts ...
Jin, Chen, Michael A, Henson
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Bioresource Technology, 2014
In this study, nanoparticles were used to enhance bioethanol production in syngas fermentation by Clostridium ljungdahlii. Six types of nanoparticles were tested: palladium on carbon, palladium on alumina, silica, hydroxyl-functionalized single-walled carbon nanotubes, alumina, and iron(III) oxide. Of these, silica nanoparticles at a concentration of 0.
Young-Kee, Kim +3 more
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In this study, nanoparticles were used to enhance bioethanol production in syngas fermentation by Clostridium ljungdahlii. Six types of nanoparticles were tested: palladium on carbon, palladium on alumina, silica, hydroxyl-functionalized single-walled carbon nanotubes, alumina, and iron(III) oxide. Of these, silica nanoparticles at a concentration of 0.
Young-Kee, Kim +3 more
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Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain
Applied Microbiology and Biotechnology, 2016A Clostridium ljungdahlii lab-isolated spontaneous-mutant strain, OTA1, has been shown to produce twice as much ethanol as the C. ljungdahlii ATCC 55383 strain when cultured in a mixotrophic medium containing fructose and syngas. Whole-genome sequencing identified four unique single nucleotide polymorphisms (SNPs) in the C.
Jason M. Whitham +7 more
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Enzyme and Microbial Technology, 2009
Effects of initial medium pH and gas flow rate on Clostridium ljungdahlii and Clostridium autoethanogenum in liquid batch, continuous gas fermentations were investigated. Synthesis gas components were supplied at varying flow rates (5, 7.5 and 10 mL/min) for C. ljungdahlii (pH 6.8 and 5.5) and C. autoethanogenum (pH 6.0).
Jacqueline L. Cotter +2 more
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Effects of initial medium pH and gas flow rate on Clostridium ljungdahlii and Clostridium autoethanogenum in liquid batch, continuous gas fermentations were investigated. Synthesis gas components were supplied at varying flow rates (5, 7.5 and 10 mL/min) for C. ljungdahlii (pH 6.8 and 5.5) and C. autoethanogenum (pH 6.0).
Jacqueline L. Cotter +2 more
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Enzyme and Microbial Technology, 2015
Acetoin reductase catalyzes the formation of 2,3-butanediol from acetoin. In Clostridium ljungdahlii DSM 13528, the gene CLJU_c23220 encoding the putative Zn(2+)-dependent alcohol dehydrogenase was cloned and expressed in Escherichia coli. The recombinant enzyme, CLAR, can catalyze the conversion of acetoin to 2,3-butanediol with NADPH as the cofactor.
Yang, Tan +3 more
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Acetoin reductase catalyzes the formation of 2,3-butanediol from acetoin. In Clostridium ljungdahlii DSM 13528, the gene CLJU_c23220 encoding the putative Zn(2+)-dependent alcohol dehydrogenase was cloned and expressed in Escherichia coli. The recombinant enzyme, CLAR, can catalyze the conversion of acetoin to 2,3-butanediol with NADPH as the cofactor.
Yang, Tan +3 more
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Clostridium ljungdahliifor production of biofuel from synthesis gas
Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 2016ABSTRACTLowering the redox potential of the growth medium is a critical step in the cultivation of obligate anaerobes for production of biofuels from synthesis gas. In this study, the simultaneous effects of reducing solutions (sodium sulfide and/or cysteine-HCl) and initial medium pH on the fermentation of synthesis gas using Clostridium ljungdahlii ...
M. Mohammadi +4 more
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Bioresource Technology, 2015
In this study, cell growth, gene expression and ethanol production were monitored under different fermentation conditions. Like its heterotrophical ABE-producing relatives, a switch from acidogenesis to solventogenesis of Clostridium ljungdahlii during the autotrophic fermentation with CO/CO2 could be observed, which occurred surprisingly in the late ...
Bin-Tao, Xie +4 more
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In this study, cell growth, gene expression and ethanol production were monitored under different fermentation conditions. Like its heterotrophical ABE-producing relatives, a switch from acidogenesis to solventogenesis of Clostridium ljungdahlii during the autotrophic fermentation with CO/CO2 could be observed, which occurred surprisingly in the late ...
Bin-Tao, Xie +4 more
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NADH gene manipulation for advancing bioelectricity in Clostridium ljungdahlii microbial fuel cells
Green Chemistry, 2016A scheme of basic metabolic pathways and energy conservation inC. ljungdahlii.
Shuo Han +3 more
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When anaerobes encounter oxygen: mechanisms of oxygen toxicity, tolerance and defence
Nature Reviews Microbiology, 2021Zheng Lu, James A Imlay
exaly
Clostridium species for fermentative hydrogen production: An overview
International Journal of Hydrogen Energy, 2021, Yanan Yin
exaly