Results 71 to 80 of about 4,511 (184)

The Cellulosome Paradigm in An Extreme Alkaline Environment

Microorganisms, 2019
Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates
Paripok Phitsuwan, Sarah Moraïs, Bareket Dassa, Bernard Henrissat, Edward A. Bayer   +4 more
doaj   +1 more source

Cellodextrin and Laminaribiose ABC Transporters in Clostridium thermocellum [PDF]

Journal of Bacteriology, 2009
ABSTRACT Clostridium thermocellum is an anaerobic thermophilic bacterium that grows efficiently on cellulosic biomass. This bacterium produces and secretes a highly active multienzyme complex, the cellulosome, that mediates the cell attachment to and hydrolysis of the crystalline cellulosic substrate.
Yakir, Nataf, Sima, Yaron, Frank, Stahl, Raphael, Lamed, Edward A, Bayer, Thomas-Helmut, Scheper, Abraham L, Sonenshein, Yuval, Shoham   +7 more
openaire   +2 more sources

Quantitative Modelling of Biohydrogen Production from Indian Agricultural Residues via Dark Fermentation

ChemistryOpen, Volume 14, Issue 5, May 2025.
This study employs quantitative modelling to assess India's biohydrogen (BioH2) potential from crop residues, revealing a 191 % production boost with pretreatment, reaching ~300 PJ annually. Key surplus‐producing states are identified, highlighting BioH2’s role in energy security and fossil fuel reduction.
Tanmay J. Deka, Ahmed I. Osman, Mohamed Farghali, Ahmed Alengebawy, Debendra C. Baruah, David W. Rooney   +5 more
wiley   +1 more source

Identification and structural characterization of a novel acetyl xylan esterase from Aspergillus oryzae

The FEBS Journal, Volume 292, Issue 10, Page 2527-2544, May 2025.
A novel acetyl xylan esterase was discovered from Aspergillus oryzae. The deacetylase activity toward wheat arabinoxylan increased synergistically with xylanase addition. The crystal structure exhibited hybrid features between two α/β‐hydrolase families, highlighting its structural uniqueness.
Chihaya Yamada, Tomoe Kato, Yoshihito Shiono, Takuya Koseki, Shinya Fushinobu   +4 more
wiley   +1 more source

Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq

Frontiers in Microbiology, 2014
The anaerobic, thermophilic bacterium, Clostridium thermocellum, secretes multi-protein enzyme complexes, termed cellulosomes, which synergistically interact with the microbial cell surface and efficiently disassemble plant cell wall biomass.
Hui eWei, Yan eFu, Lauren eMagnusson, John O. Baker, Pin-Ching eManess, Qi eXu, Shihui eYang, Andrew eBowersox, Andrew eBowersox, Igor eBogorad, Wei eWang, Melvin P. Tucker, Michael E. Himmel, Shi-You eDing   +13 more
doaj   +1 more source

Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes

ChemSusChem, Volume 18, Issue 8, April 14, 2025.
Recent advancements in the preparation of immobilized enzymes have improved both batch and continuous flow biocatalytic processes, essential for cost‐effective, sustainable production in the fine chemicals and pharmaceutical industries. This review examines advancements in carrier‐free methods, entrapment strategies, and support‐based approaches ...
Stefania Patti, Ilaria Magrini Alunno, Sara Pedroni, Sergio Riva, Erica Elisa Ferrandi, Daniela Monti   +5 more
wiley   +1 more source

Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold

Microbial Biotechnology, Volume 18, Issue 4, April 2025.
The first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Marte Elias, Kenan Meert, Julie Vanderstraeten, Babette Lamote, Yves Briers   +4 more
wiley   +1 more source

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase

Microbial Cell Factories, 2017
Background Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Result Here we examined the thermostability of eight PDCs.
Liang Tian, Skyler J. Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T. Bouvier, Adam M. Guss, Daniel G. Olson, Lee R. Lynd   +8 more
doaj   +1 more source

Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization

Engineering in Life Sciences, Volume 25, Issue 3, March 2025.
ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Gizem Ölçücü, Karl‐Erich Jaeger, Ulrich Krauss   +2 more
wiley   +1 more source

Efficient Biomass Fractionation via Organosolv for Sustainable Bioenergy Production: A Comprehensive Review

International Journal of Chemical Engineering, Volume 2025, Issue 1, 2025.
Biomass is a significant renewable feedstock for the generation of bioenergy and a gateway to a shift from fossil fuels to a renewable energy system. Efficient fractionation of biomass into the main components (cellulose, hemicellulose, and lignin) is still a problem due to its complexity.
Tesfaye Kassaw Bedru, Beteley Tekola Meshesha, Shegaw Ahmed Mohammed, Abayneh Getachew Demesa, Mani Jayakumar, Andreas Bück   +5 more
wiley   +1 more source