Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores
Nitrilases are an important class of industrial enzymes. They require mild reaction conditions and are highly efficient and environmentally friendly, so they are used to catalyze the synthesis of carboxylic acid from nitrile, a process considered ...
Chen, Zhi +7 more
core +1 more source
Training bias and sequence alignments shape protein–peptide docking by AlphaFold and related methods
Abstract Protein‐peptide interactions mediate many biological processes. Accurate structural models of protein‐peptide complexes, determined by experiment or computational prediction, are essential for understanding function and designing interaction inhibitors.
Lindsey Guan, Amy E. Keating
wiley +1 more source
Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum
Background Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C.
Ya-Jun Liu +5 more
doaj +1 more source
Biological Routes for Biohydrogen Production: A Clean and Carbon‐Free Fuel
Graphical Abstract and Lay Summary Biological routes for hydrogen (H2) production are mainly classified into four pathways: 1) direct bio‐photolysis, 2) indirect bio‐photolysis, 3) photo‐fermentation, and 4) dark fermentation. Of these pathways, dark fermentation produces the highest levels of H2 production.
Minseok Cha +2 more
wiley +1 more source
Background Clostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol.
Yang Shihui +7 more
doaj +1 more source
Regulation of Cellulase Formation in Clostridium thermocellum
Summary: True cellulase activity (i.e. degradation of crystalline cellulose) was markedly derepressed when cellobiose-grown cells were transferred to fructose or sorbitol, especially while the cells were adapting over many hours to these carbon sources. On the other hand, the long lag phase on glucose was not accompanied by derepression.
E. A. JOHNSON, F. BOUCHOT, A. L. DEMAIN
openaire +1 more source
Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum [PDF]
ABSTRACT Cellulosomes are considered to be one of the most efficient systems for the degradation of plant cell wall polysaccharides. The central cellulosome component comprises a large, noncatalytic protein subunit called scaffoldin.
Lizett, Ortiz de Ora +5 more
openaire +2 more sources
Deconstruction by C. thermocellum —from microbe mediated to dynamic redistribution of cellulosomes
By combining super-resolution imaging and machine learning to study the dynamics of biomass deconstruction by C. thermocellum , we show that this deconstruction goes from a microbe-mediated strategy to a dynamic redistribution of cellulosomes during ...
John M Yarbrough +9 more
doaj +1 more source
Grape Stalks Valorization towards Circular Economy: A Cascade Biorefinery Strategy
A cascade protocol for grape stalks valorization has been proposed, characterized by a multidisciplinary strategy using unconventional techniques and subcritical water as a green solvent, where physical/chemical treatments synergistically interact with biological treatments to obtain added value products from all the biomass components.
Carlotta Valle +11 more
wiley +1 more source
A high throughput assay of lichenase activity with Congo red dye in plants
Background Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative.
Alexander A. Tyurin +5 more
doaj +1 more source

