Results 61 to 70 of about 3,495 (163)
Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum [PDF]
Applied and Environmental Microbiology, 2017 ABSTRACT Cellulosomes are considered to be one of the most efficient systems for the degradation of plant cell wall polysaccharides. The central cellulosome component comprises a large, noncatalytic protein subunit called scaffoldin.
Lizett, Ortiz de Ora +5 more
openaire +2 more sources
Logic‐Gated HSV‐TK/GCV Suicide Gene Circuit for Triple‐Negative Breast Cancer
Advanced Science, Volume 13, Issue 20, 9 April 2026.The BRAS comprises two modular genetic components driven by distinct tumor‐specific promoters and a failsafe layer with the NOT gate. This multi‐input logic gate circuit enables precise, specific expression of HSV‐TK in breast cancer cells with hardly expression in normal cell and effectively inhibits tumor growth in a triple‐negative breast cancer ...
Shasha Tang +10 more
wiley +1 more source
Structural and biochemical properties of lichenase from Clostridium thermocellum [PDF]
Indian Journal of Microbiology, 2009 The recombinant enzyme lichenase of size 30 kDa was over-expressed using E. coli cells and purified by immobilized metal ion affinity chromatography (IMAC) and size exclusion chromatography. The enzyme displayed high activity towards lichenan and β-glucan.
Shadab, Ahmed +5 more
openaire +2 more sources
Microbial Biotechnology, Volume 19, Issue 3, March 2026.A hierarchical assembly system was established in which the coding sequences for four docking enzymes and a scaffold are co‐assembled onto a single plasmid, thereby establishing a ‘scaffoldomics’ platform for the optimisation of multi‐enzyme biosynthetic pathways.
Marte Elias +5 more
wiley +1 more source
The Cellulosome Paradigm in An Extreme Alkaline Environment
Microorganisms, 2019 Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates
Paripok Phitsuwan +4 more
doaj +1 more source
A high throughput assay of lichenase activity with Congo red dye in plants
Plant Methods, 2021 Background Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative.
Alexander A. Tyurin +5 more
doaj +1 more source
Carbohydrate Transport by the Anaerobic Thermophile Clostridium thermocellum LQRI [PDF]
Applied and Environmental Microbiology, 1995 Clostridium thermocellum is an anaerobic thermophilic bacterium which degrades cellulose and ferments the resulting glucose, cellobiose, and cellodextrins predominantly to ethanol. However, relatively little information was available on carbohydrate uptake by this bacterium. Washed cells internalized intact oligomers as large as cellopentaose.
H J, Strobel, F C, Caldwell, K A, Dawson
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Hydrogen fuel production from sugarcane and its byproducts – a critical review
Biofuels, Bioproducts and Biorefining, Volume 20, Issue 1, Page 352-376, January/February 2026.Abstract The transition to a low‐carbon economy has intensified the search for sustainable hydrogen (H2) production routes. Sugarcane and its byproducts, such as bagasse, molasses and vinasse, have emerged as promising feedstocks due to their ready availability and renewable nature. However, comprehensive evaluations of the efficiency, limitations, and
Jaqueline Calixto de Sousa +3 more
wiley +1 more source
Cereal Arabinoxylans—Their Enzymatic Degradation and Relevance for Breadmaking and Human Health
Comprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 1, January 2026.ABSTRACT As the most abundant nonstarch polysaccharides in cereals, arabinoxylans (AXs) contribute significantly to the global intake of dietary fiber. They play a crucial role in the breadmaking process with respect to dough rheology and texture, bread volume, and nutritional quality, especially when starting from wheat or rye flour.
Víctor González‐Alonso +3 more
wiley +1 more source
Regulation of Cellulase Formation in Clostridium thermocellum
Microbiology, 1985 Summary: True cellulase activity (i.e. degradation of crystalline cellulose) was markedly derepressed when cellobiose-grown cells were transferred to fructose or sorbitol, especially while the cells were adapting over many hours to these carbon sources. On the other hand, the long lag phase on glucose was not accompanied by derepression.
E. A. JOHNSON, F. BOUCHOT, A. L. DEMAIN
openaire +1 more source