Results 51 to 60 of about 3,327 (162)

Toward Efficient Cellulosic Biomass Breakdown: Enzymatic Innovation and Kinetic Insights

Macromolecular Materials and Engineering, Volume 311, Issue 6, June 2026.
By combining a processive GH7, CBM2a, and GH3 into single enzyme, the GCG fusion protein effectively breaks down crystalline cellulose. This tri‐domain architecture reduces cellobiose inhibition at the cellulose surface allowing for the simultaneous targeting of the substrate, depolymerization, and removal of products.
Anoth Maharjan, Mi‐Reu Kim, Hyoseon Kim, Jungoh Ahn, Jung‐Ho Park   +4 more
wiley   +1 more source

Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7 [PDF]

Genome Announcements, 2016
ABSTRACT We and others have shown the utility of long sequence reads to improve genome assembly quality. In this study, we generated PacBio DNA sequence data to improve the assemblies of draft genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and ...
Sagar M. Utturkar, Edward A. Bayer, Ilya Borovok, Raphael Lamed, Richard A. Hurt, Miriam L. Land, Dawn M. Klingeman, Dwayne Elias, Jizhong Zhou, Marcel Huntemann, Alicia Clum, Manoj Pillay, Krishnaveni Palaniappan, Neha Varghese, Natalia Mikhailova, Dimitrios Stamatis, T. B. K. Reddy, Chew Yee Ngan, Chris Daum, Nicole Shapiro, Victor Markowitz, Natalia Ivanova, Nikos Kyrpides, Tanja Woyke, Steven D. Brown   +24 more
openaire   +4 more sources

Decoding Biomass-Sensing Regulons of Clostridium thermocellum Alternative Sigma-I Factors in a Heterologous Bacillus subtilis Host System.

PLoS ONE, 2016
The Gram-positive, anaerobic, cellulolytic, thermophile Clostridium (Ruminiclostridium) thermocellum secretes a multi-enzyme system called the cellulosome to solubilize plant cell wall polysaccharides.
Iván Muñoz-Gutiérrez, Lizett Ortiz de Ora, Inna Rozman Grinberg, Yuval Garty, Edward A Bayer, Yuval Shoham, Raphael Lamed, Ilya Borovok   +7 more
doaj   +1 more source

Harnessing microbial power to degrade hydrocarbon‐based plastics

mLife, Volume 5, Issue 3, Page 263-281, June 2026.
Abstract The growing global plastic waste crisis demands the development of urgent, effective, and sustainable solutions. While conventional recycling methods present intrinsic limitations, microbial biodegradation of plastic waste has emerged as a promising alternative.
Hui Li, Xuanyu Tao, Lina Sun, Aifen Zhou, Jizhong Zhou   +4 more
wiley   +1 more source

Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum [PDF]

Applied and Environmental Microbiology, 2017
ABSTRACT Cellulosomes are considered to be one of the most efficient systems for the degradation of plant cell wall polysaccharides. The central cellulosome component comprises a large, noncatalytic protein subunit called scaffoldin.
Lizett, Ortiz de Ora, Iván, Muñoz-Gutiérrez, Edward A, Bayer, Yuval, Shoham, Raphael, Lamed, Ilya, Borovok   +5 more
openaire   +2 more sources

Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum

Biotechnology for Biofuels, 2018
Background Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C.
Ya-Jun Liu, Shiyue Liu, Sheng Dong, Renmin Li, Yingang Feng, Qiu Cui   +5 more
doaj   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, Volume 16, Issue 4, Page 653-669, April 2026.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Deconstruction by C. thermocellum —from microbe mediated to dynamic redistribution of cellulosomes

Life Science Alliance
By combining super-resolution imaging and machine learning to study the dynamics of biomass deconstruction by C. thermocellum , we show that this deconstruction goes from a microbe-mediated strategy to a dynamic redistribution of cellulosomes during ...
John M Yarbrough, Neal N Hengge, Qi Xu, Samantha J Ziegler, Daehwan Chung, Shu Huang, Sarah Moraïs, Itzhak Mizrahi, Edward A Bayer, Yannick J Bomble   +9 more
doaj   +1 more source

Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress

BMC Genomics, 2012
Background Clostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol.
Yang Shihui, Giannone Richard J, Dice Lezlee, Yang Zamin K, Engle Nancy L, Tschaplinski Timothy J, Hettich Robert L, Brown Steven D   +7 more
doaj   +1 more source

Logic‐Gated HSV‐TK/GCV Suicide Gene Circuit for Triple‐Negative Breast Cancer

Advanced Science, Volume 13, Issue 20, 9 April 2026.
The BRAS comprises two modular genetic components driven by distinct tumor‐specific promoters and a failsafe layer with the NOT gate. This multi‐input logic gate circuit enables precise, specific expression of HSV‐TK in breast cancer cells with hardly expression in normal cell and effectively inhibits tumor growth in a triple‐negative breast cancer ...
Shasha Tang, Yuan Fang, Lingli Jin, Dongyang Liu, Yicheng Liu, Ruijia Zheng, Liyun Yong, Xin Wu, Longliang Qiao, Meiyan Wang, Fengfeng Cai   +10 more
wiley   +1 more source