Results 51 to 60 of about 3,495 (163)

Integrative SAXS and AFM analysis of engineered carbohydrate‐active enzyme assemblies with tunable spatial organization

Protein Science, Volume 35, Issue 7, July 2026.
Abstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Iker Pardo Larrabeiti, Manuel Eibinger, Jeremy Esque, Stéphanie Pradeau, Sébastien Fort, Sarah Moraïs, Itzhak Mizrahi, Edward A. Bayer, Bernd Nidetzky, Cédric Y. Montanier, Pierre Roblin, Claire Dumon   +11 more
wiley   +1 more source

Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose‐Degrading Anaerobic Bacteria

Angewandte Chemie, Volume 137, Issue 24, June 10, 2025.
The structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Keishi Ishida, Jana Krabbe, Philippe R. Meisinger, Gulimila Shabuer, Sebastian Schieferdecker, Michael Cyrulies, Cedric Tank, Emma Barnes, Christian Paetz, Christian Hertweck   +9 more
wiley   +2 more sources

Toward Efficient Cellulosic Biomass Breakdown: Enzymatic Innovation and Kinetic Insights

Macromolecular Materials and Engineering, Volume 311, Issue 6, June 2026.
By combining a processive GH7, CBM2a, and GH3 into single enzyme, the GCG fusion protein effectively breaks down crystalline cellulose. This tri‐domain architecture reduces cellobiose inhibition at the cellulose surface allowing for the simultaneous targeting of the substrate, depolymerization, and removal of products.
Anoth Maharjan, Mi‐Reu Kim, Hyoseon Kim, Jungoh Ahn, Jung‐Ho Park   +4 more
wiley   +1 more source

Decoding Biomass-Sensing Regulons of Clostridium thermocellum Alternative Sigma-I Factors in a Heterologous Bacillus subtilis Host System.

PLoS ONE, 2016
The Gram-positive, anaerobic, cellulolytic, thermophile Clostridium (Ruminiclostridium) thermocellum secretes a multi-enzyme system called the cellulosome to solubilize plant cell wall polysaccharides.
Iván Muñoz-Gutiérrez, Lizett Ortiz de Ora, Inna Rozman Grinberg, Yuval Garty, Edward A Bayer, Yuval Shoham, Raphael Lamed, Ilya Borovok   +7 more
doaj   +1 more source

Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum

Biotechnology for Biofuels, 2018
Background Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C.
Ya-Jun Liu, Shiyue Liu, Sheng Dong, Renmin Li, Yingang Feng, Qiu Cui   +5 more
doaj   +1 more source

Harnessing microbial power to degrade hydrocarbon‐based plastics

mLife, Volume 5, Issue 3, Page 263-281, June 2026.
Abstract The growing global plastic waste crisis demands the development of urgent, effective, and sustainable solutions. While conventional recycling methods present intrinsic limitations, microbial biodegradation of plastic waste has emerged as a promising alternative.
Hui Li, Xuanyu Tao, Lina Sun, Aifen Zhou, Jizhong Zhou   +4 more
wiley   +1 more source

Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress

BMC Genomics, 2012
Background Clostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol.
Yang Shihui, Giannone Richard J, Dice Lezlee, Yang Zamin K, Engle Nancy L, Tschaplinski Timothy J, Hettich Robert L, Brown Steven D   +7 more
doaj   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, Volume 16, Issue 4, Page 653-669, April 2026.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Deconstruction by C. thermocellum —from microbe mediated to dynamic redistribution of cellulosomes

Life Science Alliance
By combining super-resolution imaging and machine learning to study the dynamics of biomass deconstruction by C. thermocellum , we show that this deconstruction goes from a microbe-mediated strategy to a dynamic redistribution of cellulosomes during ...
John M Yarbrough, Neal N Hengge, Qi Xu, Samantha J Ziegler, Daehwan Chung, Shu Huang, Sarah Moraïs, Itzhak Mizrahi, Edward A Bayer, Yannick J Bomble   +9 more
doaj   +1 more source

Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum

Biotechnology for Biofuels, 2017
Background Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and ...
Jie Zhang, Shiyue Liu, Renmin Li, Wei Hong, Yan Xiao, Yingang Feng, Qiu Cui, Ya-Jun Liu   +7 more
doaj   +1 more source