Results 31 to 40 of about 3,327 (162)

Transcriptomic Analysis of Clostridium thermocellum in Cellulolytic Consortium after Artificial Reconstruction to Enhance Ethanol Production

open access: yesBioResources, 2015
The cellulolytic and ethanologenic bacterial community is a promising candidate for the production of bioethanol from lignocellulose. In this study, by artificially changing the ratio of Clostridium thermocellum in the cellulolytic consortium H, ethanol ...
Hongyu Li   +5 more
doaj   +1 more source

Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs)

open access: yesMetabolic Engineering Communications, 2016
Clostridium thermocellum is a promising candidate for ethanol production from cellulosic biomass, but requires metabolic engineering to improve ethanol yield.
Shuen Hon   +6 more
doaj   +1 more source

Nanoscale resolution of microbial fiber degradation in action

open access: yeseLife, 2022
The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology.
Meltem Tatli   +6 more
doaj   +1 more source

Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics

open access: yesBiotechnology for Biofuels, 2018
Background Clostridium (Ruminiclostridium) thermocellum is a model fermentative anaerobic thermophile being studied and engineered for consolidated bioprocessing of lignocellulosic feedstocks into fuels and chemicals. Engineering efforts have resulted in
Jason M. Whitham   +9 more
doaj   +1 more source

Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostridium thermohydrosulfuricum [PDF]

open access: yesJournal of Bacteriology, 1982
Clostridium thermohydrosulfuricum consumed glucose in preference to cellobiose as an energy source for growth. The rates of substrate uptake in glucose- and cellobiose-grown cell suspensions were 45 and 24 nmol/min per mg (dry weight), respectively, at 65 degrees C.
T K, Ng, J G, Zeikus
openaire   +2 more sources

Thermostable chaperonin from Clostridium thermocellum [PDF]

open access: yesBiochemical Journal, 1996
Homologues of the chaperonins Cpn60 and Cpn10 have been purified from the Gram-positive cellulolytic thermophile Clostridium thermocellum. The Cpn60 protein was purified by ATP-affinity chromatography and the Cpn10 protein was purified by gel-filtration, ion-exchange and hydrophobic interaction chromatographies.
S J, Cross   +4 more
openaire   +2 more sources

Tracking the cellulolytic activity of Clostridium thermocellum biofilms [PDF]

open access: yesBiotechnology for Biofuels, 2013
Background Microbial cellulose conversion by Clostridium thermocellum 27405 occurs predominantly through the activity of substrate-adherent bacteria organized in thin, primarily single cell-layered biofilms. The importance of cellulosic surface exposure to microbial hydrolysis has received little attention despite its implied impact on conversion ...
Dumitrache, Alexandru   +4 more
openaire   +4 more sources

Glycolysis without pyruvate kinase in Clostridium thermocellum [PDF]

open access: yesMetabolic Engineering, 2017
The metabolism of Clostridium thermocellum is notable in that it assimilates sugar via the EMP pathway but does not possess a pyruvate kinase enzyme. In the wild type organism, there are three proposed pathways for conversion of phosphoenolpyruvate (PEP) to pyruvate, which differ in their cofactor usage. One path uses pyruvate phosphate dikinase (PPDK),
Olson DG   +9 more
openaire   +3 more sources

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature

open access: yesMetabolic Engineering Communications, 2016
Clostridium thermocellum is a leading candidate for the consolidated bioprocessing of lignocellulosic biomass for the production of fuels and chemicals.
Joseph Groom   +5 more
doaj   +1 more source

A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

open access: yesBMC Microbiology, 2011
Background Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C.
Fan Keqiang   +6 more
doaj   +1 more source

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